論文 - 佐原 健
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Kageyama D, Ohno M, Sasaki T, Yoshido A, Konagaya T, Jouraku A, Kuwazaki S, Kanamori H, Katayose Y, Narita S, Miyata M, Riegler M, Sahara K
Evolution Letters 1 ( 5 ) 232 - 244 2017年10月 [査読有り]
国際的学術誌 共著・分担
(2017): 1(5): 232-244.
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Relative humidity and maintenance of p50 silkworms reared on artificial diet.
Yuri K, Sahara K
Journal of Insect Biotechnology and Sericology 86 43 - 47 2017年04月 [査読有り]
学会誌 共著・分担
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A history of chromosome identification in Bombyx mori
Ken Sahara, Atsuo Yoshido, Yuji Yasukochi
Chromosome Science 19 3 - 9 2017年04月 [査読有り]
学会誌 共著・分担
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Variation in nesting behavior of eight species of spider mites, Stigmaeopsis having sociality
Yutaka Saito, Yan-Xuan Zhang, Kotaro Mori, Katsura Ito, Yukie Sato, Anthony R. Chittenden, Jian-Zhen Lin, Younghae Chae, Takane Sakagami, Ken Sahara
Science of Nature 103 87 2016年10月 [査読有り]
学術誌 共著・分担
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Fissions, fusions, and translocations shaped the karyotype and multiple sex chromosome constitution of the northeast-Asian wood white butterfly, Leptidea amurensis
JINDRA SICHOVA, MIZUKI OHNO, VLAD DINCA, MICHIHITO WATANABE, KEN SAHARA, FRANTISEK MAREC
Biological Journal of the Linnean Society 118 457 - 471 2016年05月 [査読有り]
学術誌 共著・分担
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The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction
A Yoshido, F Marec, K Sahara
Heredity 116 424 - 433 2016年04月 [査読有り]
学術誌 共著・分担
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XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima
Jack E. Green, Martina Dalíková, Ken Sahara, František Marec, Michael Akam
PLoSONE 11 ( 2 ) e0150292 2016年02月 [査読有り]
学術誌 共著・分担
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Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.
Ito K, Katsuma S, Kuwazaki S, Jouraku A, Fujimoto T, Sahara K, Yasukochi Y, Yamamoto K, Tabunoki H, Yokoyama T, Kadono-Okuda K, Shimada T
Heredity 116 52 - 59 2016年01月 [査読有り]
国際的学術誌 共著・分担
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A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm.
Yasukochi Y, Ohno M, Shibata F, Jouraku A, Nakano R, Ishikawa Y, Sahara K
Heredity 116 75 - 83 2016年01月 [査読有り]
国際的学術誌 共著・分担
(2016): Heredity 116, 75-83
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Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies.
Šíchová J, Voleníková A, Dincă V, Nguyen P, Vila R, Sahara K, Marec F
BMC Evolutionary Biology 15 ( 89 ) 2015年05月 [査読有り]
国際的学術誌 共著・分担
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A new molecular technique for determining the sex of Harmonia axyridis.
Gotoh H, Nishikawa H, Sahara K, Yaginuma T, Niimi T
Journal of Insect Biotechnology and Sericology 84 9 - 15 2015年02月 [査読有り]
学会誌 共著・分担
We have developed a fast and efficient PCR-based technique to sex all developmental stages of the ladybird beetle Harmonia axyridis. Previously, we established a male-specific fluorescent expressing strain of H. axyridis with the piggyBac vector. FISH analyses revealed that the transgene inserted into the H. axyridis genome in the male Y-chromosome. Because only males expressed the fluorescent signal, we were able to differentiate between the sexes of this transgenic strain even in newly hatched larvae. We took advantage of this insertion and designed an inverse PCR reaction and genome walking surrounding the inserted region to identify 3,781 bp of the male Y-chromosome genomic sequence. From this male specific region, we designed a PCR-based protocol to identify sexes from all H. axyridis developmental stages.
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FISH identification of Helicoverpa armigera and Mamestra brassicae chromosomes by BAC and fosmid probes.
Sahara K , Yoshido A, Shibata F, Fujikawa N, Okabe T, Tanaka-Okuyama M, Yasukochi Y
Insect Biochemistry and Molecular Biology in press 2013年04月 [査読有り]
国際的学術誌 共著・分担
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Neo-sex chromosomes and adaptive potential in tortricid pests.
Nguyen P, Sýkorová M, Šíchová J, Kůta V, Dalíková M, Čapková Frydrychová R, Neven LG, Sahara K, Marec F
The Proceedings National Academy of Sciences USA 110 6931 - 6936 2013年04月 [査読有り]
国際的学術誌 共著・分担
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Rapid turnover of the W chromosome in geographical populations of wild silkmoths, Samia cynthia ssp.
Yoshido A, Šíchová J, Kubíčková S, Marec F, Sahara K
Chromosome Research 21 149 - 164 2013年04月 [査読有り]
国際的学術誌 共著・分担
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EST sequencing and fosmid library construction in a non-model moth, Mamestra brassicae, for comparative mapping （訳）比較マッピングのための非モデルガ類のヨトウガにおけるEST配列決定とフォスミドライブラリー作製
Kamimura M, Taetishi K, Tanaka-Okuyama M, Okabe T, Shibata F, Sahara K, Yasukochi Y
Genome in press 2012年10月 [査読有り]
国際的学術誌 共著・分担
ガとチョウ類のFISHを通じた比較ゲノム解析を進めるため、重要な農業害虫であるヨトウガのフォスミドライブラリーを作製し、EST配列の決定からカイコ単一遺伝子のオルソログを含むヨトウガフォスミドを120個特定した。この研究ではcDNAの行列プールを用いることでEST配列決定の効率化を実現した。一部のフォスミドを用いたFISH解析よりカイコとのシンテニーが示された。
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Phenotypic grouping of 141 BmNPVs lacking viral gene sequences （訳）ウイルス遺伝子欠損による141BmNPV遺伝子の形質グルーピング
Ono C, Kamagata T, Taka H, Sahara K, Asano S, Bando H
Virus Research 165 ( 2 ) 197 - 206 2012年05月 [査読有り]
国際的学術誌 共著・分担
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Sex chromosome evolution in moths and butterflies （訳）ガとチョウ類における性染色体進化
Sahara K, Yoshido A, Traut W
Chromosome Research 20 ( 1 ) 83 - 94 2011年12月 [査読有り]
国際的学術誌 共著・分担
ガとチョウの仲間である鱗翅目昆虫の雌へテロ型(WZ/ZZ)性染色体進化についての総説。FISHによる性染色体同定、性染色体の由来、性染色体構成の多様性、性決定、性染色体上の遺伝子、遺伝子量補正、性染色体工学による応用ならびに展望に関して我々の研究データを中心に概説してある。
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Yasukochi Y, Miura N, Nakano R, Sahara K, Ishikawa Y
PLoS ONE 6 ( 4 ) e18843 2011年04月 [査読有り]
国際的学術誌 共著・分担
This is the first report of tandemly arrayed sex pheromone receptor genes in Lepidoptera. The localization of an OR gene cluster on the Z chromosome agrees with previous findings for a Z-linked locus responsible for O. nubilalis male behavioral response to sex pheromone. The 181-bp irect repeats might enhance gene duplications by unequal crossovers. An autosomal locus responsible for male esponse to sex pheromone in Heliothis virescens and H.subflexa was recently reported to contain at least four OR genes. Taken together, these findings support the hypothesis that eneration of additional copies of OR genes can increase the potential for male moths to acquire altered specificity for pheromone components, and accordingly, facilitate differentiation of sex pheromones.
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Samia cynthia versus Bombyx mori: Comparative gene mapping between a species with a low-number karyotype and the model species of Lepidoptera. （訳）シンジュサン対カイコ:鱗翅目昆虫における染色体数の少ない種とモデル種の比較遺伝子マッピング
Yoshido A, Yasukochi Y, Sahara K
Insect Biochemistry and Molecular Biology 41 370 - 377 2011年03月 [査読有り]
国際的学術誌 共著・分担
We performed gene-based comparative FISH mapping between a wild silkmoth, Samia cynthia ssp. with a low number of chromosomes (2n=25-28) and the model species, Bombyx mori (2n=56), in order to identify the genomic components that make up the chromosomes in a low-number karyotype. Mapping
of 64 fosmid probes containing orthologs of B. mori genes revealed that the homologues of either two or four B. mori chromosomes constitute the S. c. ricini (Vietnam opulation, 2n=27/28, Z0/ZZ) autosomes. Where tested, even the gene order was conserved between S. c. ricini and B. mori. This was also true for the originally autosomal parts of the neo-sex chromosomes in S. c. walkeri (Sapporo population, 2n=26/26, neo-Wneo-Z/neo-Zneo-Z) and S. cynthia subsp. indet. (Nagano population, 2n=25/26, neo-WZ1Z2/Z1Z1Z2Z2). The results are evidence for an internal stability of lepidopteran chromosomes even when all autosomes had undergone fusion processes to form a low-number karyotype. -
Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K
Journal of Biomedicine and Biotechnology ID 165894 2010年11月 [査読有り]
国際的学術誌 共著・分担
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran.
A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species.