論文 - 佐原　健

Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae).

Fujimoto T, Okumura A, Yoshido A, Yasukochi Y, Suzuki G, Sahara K

. Journal of Insect Biotechnology and Sericology   87 ( 2 ) 61 - 69   2018年05月  [査読有り]

学会誌  共著・分担


We constructed the first trichopteran bacterial artificial chromosome (BAC) library in Stenopsyche marmorata. The BAC library consists of 32,256 clones with an average insert size of 65.38 kb. We also made a cDNA library from an S. marmorata adult male and determined 598 unique sequences. Using the obtained sequences we designed putative single tagged site (STS) primers and selected 81 BACs containing orthologs of Bombyx mori genes by three-step PCR selection method. These BACs will be used for gene-based comparative mapping by fluorescence in situ hybridization (FISH) to identify regions of conserved synteny between the trichopteran and lepidopteran genomes.

Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of distantly related ditrysian Lepidoptera.

Yasukochi Y, Yang B, Fujimoto T, SaharaK, Matsuo T, Ishikawa Y

PLoS ONE   13 ( 2 ) e0192762   2018年02月  [査読有り]

国際的学術誌  共著・分担

DOI

Inheritance of cocoon color mutation and heterosis in Antheraea yamamai.

Yamada Y, Asano S, Bando H, Sahara K

Journal of Wild Silkworm & Silk   20   57 - 61   2017年10月  [査読有り]

国際的学術誌  共著・分担

Antheraea yamamai is a wild silkmoth that spins yellow-green silk to produce cocoons. Cocoons that are less yellowish in color, referred to as emerald green (EG), have been found in A. yamamai mass-rearing for cocoon production. Here, we examined EG inheritance by crossing individuals that produce EG cocoons with those that produce normal-colored (normal green: NG) cocoons. F1 progenies from the reciprocal cross between EG and NG individuals mostly produced NG cocoons. In the backcross, color
segregation mostly indicated that the EG mutation is controlled by a single recessive gene locus. Single-pair selection of cocoon weight for 4 years revealed that there is a heterosis effect on cocoon weight in the hybrid
progenies.

Feminizing Wolbachia endosymbiont disrupts maternal sex chromosome inheritance in a butterfly species.

Kageyama D, Ohno M, Sasaki T, Yoshido A, Konagaya T, Jouraku A, Kuwazaki S, Kanamori H, Katayose Y, Narita S, Miyata M, Riegler M, Sahara K

Evolution Letters   1 ( 5 ) 232 - 244   2017年10月  [査読有り]

国際的学術誌  共著・分担

(2017): 1(5): 232-244.

Relative humidity and maintenance of p50 silkworms reared on artificial diet.

Yuri K, Sahara K

Journal of Insect Biotechnology and Sericology   86   43 - 47   2017年04月  [査読有り]

学会誌  共著・分担

A history of chromosome identification in Bombyx mori

Ken Sahara, Atsuo Yoshido, Yuji Yasukochi

Chromosome Science   19   3 - 9   2017年04月  [査読有り]

学会誌  共著・分担

Variation in nesting behavior of eight species of spider mites, Stigmaeopsis having sociality

Yutaka Saito, Yan-Xuan Zhang, Kotaro Mori, Katsura Ito, Yukie Sato, Anthony R. Chittenden, Jian-Zhen Lin, Younghae Chae, Takane Sakagami, Ken Sahara

Science of Nature   103   87   2016年10月  [査読有り]

学術誌  共著・分担

Fissions, fusions, and translocations shaped the karyotype and multiple sex chromosome constitution of the northeast-Asian wood white butterfly, Leptidea amurensis

JINDRA SICHOVA, MIZUKI OHNO, VLAD DINCA, MICHIHITO WATANABE, KEN SAHARA, FRANTISEK MAREC

Biological Journal of the Linnean Society   118   457 - 471   2016年05月  [査読有り]

学術誌  共著・分担

The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction

A Yoshido, F Marec, K Sahara

Heredity   116   424 - 433   2016年04月  [査読有り]

学術誌  共著・分担

XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima

Jack E. Green, Martina Dalíková, Ken Sahara, František Marec, Michael Akam

PLoSONE   11 ( 2 ) e0150292   2016年02月  [査読有り]

学術誌  共著・分担

DOI

Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.

Ito K, Katsuma S, Kuwazaki S, Jouraku A, Fujimoto T, Sahara K, Yasukochi Y, Yamamoto K, Tabunoki H, Yokoyama T, Kadono-Okuda K, Shimada T

Heredity   116   52 - 59   2016年01月  [査読有り]

国際的学術誌  共著・分担

A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm.

Yasukochi Y, Ohno M, Shibata F, Jouraku A, Nakano R, Ishikawa Y, Sahara K

Heredity   116   75 - 83   2016年01月  [査読有り]

国際的学術誌  共著・分担

(2016): Heredity 116, 75-83

Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies.

Šíchová J, Voleníková A, Dincă V, Nguyen P, Vila R, Sahara K, Marec F

BMC Evolutionary Biology   15 ( 89 )   2015年05月  [査読有り]

国際的学術誌  共著・分担

A new molecular technique for determining the sex of Harmonia axyridis.

Gotoh H, Nishikawa H, Sahara K, Yaginuma T, Niimi T

Journal of Insect Biotechnology and Sericology   84   9 - 15   2015年02月  [査読有り]

学会誌  共著・分担

We have developed a fast and efficient PCR-based technique to sex all developmental stages of the ladybird beetle Harmonia axyridis. Previously, we established a male-specific fluorescent expressing strain of H. axyridis with the piggyBac vector. FISH analyses revealed that the transgene inserted into the H. axyridis genome in the male Y-chromosome. Because only males expressed the fluorescent signal, we were able to differentiate between the sexes of this transgenic strain even in newly hatched larvae. We took advantage of this insertion and designed an inverse PCR reaction and genome walking surrounding the inserted region to identify 3,781 bp of the male Y-chromosome genomic sequence. From this male specific region, we designed a PCR-based protocol to identify sexes from all H. axyridis developmental stages.

FISH identification of Helicoverpa armigera and Mamestra brassicae chromosomes by BAC and fosmid probes.

Sahara K , Yoshido A, Shibata F, Fujikawa N, Okabe T, Tanaka-Okuyama M, Yasukochi Y

Insect Biochemistry and Molecular Biology   in press   2013年04月  [査読有り]

国際的学術誌  共著・分担

Neo-sex chromosomes and adaptive potential in tortricid pests.

Nguyen P, Sýkorová M, Šíchová J, Kůta V, Dalíková M, Čapková Frydrychová R, Neven LG, Sahara K, Marec F

The Proceedings National Academy of Sciences USA   110   6931 - 6936   2013年04月  [査読有り]

国際的学術誌  共著・分担

Rapid turnover of the W chromosome in geographical populations of wild silkmoths, Samia cynthia ssp.

Yoshido A, Šíchová J, Kubíčková S, Marec F, Sahara K

Chromosome Research   21   149 - 164   2013年04月  [査読有り]

国際的学術誌  共著・分担

EST sequencing and fosmid library construction in a non-model moth, Mamestra brassicae, for comparative mapping （訳）比較マッピングのための非モデルガ類のヨトウガにおけるEST配列決定とフォスミドライブラリー作製

Kamimura M, Taetishi K, Tanaka-Okuyama M, Okabe T, Shibata F, Sahara K, Yasukochi Y

Genome   in press   2012年10月  [査読有り]

国際的学術誌  共著・分担

ガとチョウ類のFISHを通じた比較ゲノム解析を進めるため、重要な農業害虫であるヨトウガのフォスミドライブラリーを作製し、EST配列の決定からカイコ単一遺伝子のオルソログを含むヨトウガフォスミドを120個特定した。この研究ではcDNAの行列プールを用いることでEST配列決定の効率化を実現した。一部のフォスミドを用いたFISH解析よりカイコとのシンテニーが示された。

Phenotypic grouping of 141 BmNPVs lacking viral gene sequences （訳）ウイルス遺伝子欠損による141BmNPV遺伝子の形質グルーピング

Ono C, Kamagata T, Taka H, Sahara K, Asano S, Bando H

Virus Research   165 ( 2 ) 197 - 206   2012年05月  [査読有り]

国際的学術誌  共著・分担

Sex chromosome evolution in moths and butterflies （訳）ガとチョウ類における性染色体進化

Sahara K, Yoshido A, Traut W

Chromosome Research   20 ( 1 ) 83 - 94   2011年12月  [査読有り]

国際的学術誌  共著・分担

ガとチョウの仲間である鱗翅目昆虫の雌へテロ型(WZ/ZZ)性染色体進化についての総説。FISHによる性染色体同定、性染色体の由来、性染色体構成の多様性、性決定、性染色体上の遺伝子、遺伝子量補正、性染色体工学による応用ならびに展望に関して我々の研究データを中心に概説してある。