論文 - 佐原 健
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錦秋×鍾和において5齢幼虫脱皮個体を得るアラテクトミー方法
KIM Jung, 金児 雄, 佐原 健,
東北蚕糸・昆虫利用研究報告 49 13 - 17 2024年12月 [査読有り]
その他（含・紀要） 共著・分担
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Diogo Cavalcanti Cabral-de-Mello1, Atsuo Yoshido, Diogo Milani, Jindra Šíchová, Ken Sahara, František Marec
DNA Research ( Oxford University Press ) 2024年10月 [査読有り]
学術誌 単著
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W chromosome sequences of two bombycid moths provide an insight into the origin of Fem
Jung Lee, Toshiaki Fujimoto, Katsushi Yamaguchi, Shuji Shigenobu, Ken Sahara, Atsushi Toyoda, Toru Shimada
Molecular Ecology 33 e17434 2024年06月 [査読有り]
学術誌 単著
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人工飼料育によるボルバキア感染ならびに非感染キタキチョウの生長比較
佐原 健, 菊池 将騎, 吉田 理央, 飯沼 椋詞, 村山 蒼悟, 陰山 大輔
東北蚕糸・昆虫利用研究報告 48 22 - 25 2023年12月 [査読有り]
その他（含・紀要） 共著・分担
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Male-killing virus in a noctuid moth Spodoptera litura
Keisuke Nagamine, Yoshiaki Kanno, Ken Sahara, Toshiaki Fujimoto, Atsuo Yoshido, Yukio Ishikawae, Misato Teraoa, Daisuke Kageyamab, Yoshinori Shintania
PNAS ( National Academy of Sciences of the United States of America ) 120 ( 46 ) e2312124120 - e2312124120 2023年11月 [査読有り]
学術誌 共著・分担
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Lepidopteran Synteny Units reveal deep chromosomal conservation in butterflies and moths
Walther Traut, Ken Sahara, Richard H. ffrench-Constant
G3 ( Oxford ) 13 ( 8 ) jkad134 - jkad134 2023年06月 [査読有り]
学術誌 共著・分担
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竹内 悠真・船木 颯斗・佐原 健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 47 1 - 6 2022年12月 [査読有り]
その他（含・紀要） 共著・分担
2021年と2022年に盛岡市内の数地点でキタキチョウを採集し、ボルバキア感染状況を調査した。さらに、昨年度報告した、判別用プライマー（竹内・佐原, 2021）を用いて、感染個体と非感染個体におけるミトコンドリアの由来の特定を試みた。
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18S rDNAを含むオオモンシロチョウBACクローンの発見と利用
依田直哉・藤本章晃・佐原健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 47 7 - 10 2022年12月 [査読有り]
その他（含・紀要） 共著・分担
rDNAクラスターの染色体上の位置は、rDNAを含むプローブを用いて染色体標本にFISH（fluorescence in situ hybridization）を行うことで視覚的に確認できる。特に18S rDNAは配列保存性が高いため、コドリンガ（Cydia pomonella）のgDNAを用いたPCRにより18S rDNAを含む1,650 bpの配列を増幅し、増幅産物にラベルしたプローブが広く利用されている（FUKOVÁ et al. 2005）。本研究では、18S rDNAを含むタンデムrDNA配列がクローンされたと考えられるオオモンシロチョウBACクローンを発見したので、他種へのrDNAクラスター検出に用いることが可能かどうか検証した。
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依田直哉・北島博・鈴木剛・藤本章晃・安河内祐二・佐原健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 46 1 - 5 2021年12月 [査読有り]
その他（含・紀要） 共著・分担
OKUMURA et al.（2019）が構築したコウモリガBACライブラリーの総インサートサイズは約2 Gbと推定される。ゲノムリダンダンシーを考慮すると、小林（未発表）の選抜した、カイコ単一遺伝子オルソログを含むコウモリガBACクローンが69に留まったのは、ライブラリーのゲノムカバー率の低さが影響した可能性も否めない。染色体の対応関係を網羅的に比較するためには、1染色体あたり4以上のマーカーを必要とする。小林（未発表）では、カイコ単一オルソログ含むBACを複数獲得できていない染色体が存在し、研究完遂にはさらなるBAC選抜が必要と考えられた。そこで、本研究では新規のカイコオルソログ含有コウモリガBACを効率的に選抜するために新規コウモリガBACライブラリーの構築を行った。
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小原聡大・竹村洋子・酒井弘貴・佐原健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 46 12 - 16 2021年12月 [査読有り]
その他（含・紀要） 共著・分担
SxlIn3-homoの有核精子に異常がないことは、3倍体雄との重複交尾により確認された。そのため、この方法による系統維持は可能である。しかし、系統維持の度毎に3倍体を誘起することはスペースや労力的に負担が大きかった。そこで、黒縞遺伝子座（pS）をホモにもつre9系統をヘルパーとして利用する方法が開発された（SAKAI et al. 2020）。この研究では、卵色を用いたre9の交尾時間の最適化とSxlIn3-homo雌への確認実験を行った。最適な重複交尾の結果、SxlIn3-homo個体と考えられる次世代の形蚕ホモ（+p/+p）個体割合は0～31.5％、最大受精卵数は112個であった。この研究での実験区数は6と小さく、形蚕個体の受精については確認されていなかった。そこで本研究では、これら次世代の単交尾における受精率がゼロであるSxlIn3-homoだと証明するとともに、その次世代へも系統維持できることを示す目的で実験を行った。
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竹内悠真・佐原健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 46 6 - 11 2021年12月 [査読有り]
その他（含・紀要） 共著・分担
非感染キタキチョウの由来の特定は、ミトコンドリア配列を確認すればよい（NARITA et al. 2006; MIYATA et al. 2017; 2020）。しかし、大量の個体を配列特定するにはコストがかかる。そこで本研究では、ミトコンドリア遺伝子配列がキタキチョウ型かミナミキチョウ型かを簡便に識別できるPCR法の確立に取り組んだ。先行研究（NARITA et al. 2006）で公表されているキタキチョウおよびミナミキチョウのND5配列を参考にプライマーを設計し、キタキチョウ型とミナミキチョウ型の簡易識別に成功した。
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藤本章晃・大野瑞紀 ・佐原健
東北蚕糸・昆虫利用研究報告 ( 日本蚕糸学会東北支部 ) 45 10 - 13 2020年12月 [査読有り]
その他（含・紀要） 共著・分担
S. tienmushanensis（St）のゲノムデータについてヒゲナガカワトビケラ染色体上へのオルソログマッピングを通じた染色体レベルのゲノムアセンブルを目指した。本研究では、Stコンティグデータとヒゲナガカワトビケラの配列情報を対応付けるために、ヒゲナガカワトビケラ蛹個体から抽出した全RNAを用いてRNAseqを行った。さらに、獲得した配列情報を用いてカイコ遺伝子と1対1対応のオルソログもしくはStコンティグデータに対応する配列を持つヒゲナガカワトビケラのBACセレクションを行った。
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Double-copulated introduction of ejaculate with dominant larval phenotype to maintain Bombyx mori mutant with dysfunctional apyrene sperm.
Sakai H, Konagaya T, Takemura Y, Sahara K, Niimi T
Journal of Insect Biotechnology and Sericology 89 ( 1 ) 39 - 43 2020年04月 [査読有り]
学術誌 単著
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Development of an application, Bombyx mori tool for ortholog picking 1 (BmTOP).
Ohno M, Kawamoto M, Sahara K
Journal of Insect Biotechnology and Sericology 89 ( 1 ) 1 - 7 2020年02月 [査読有り]
学会誌 共著・分担
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BAC selection of the large white butterfly, Pieris brassicae (Lepidoptera: Pieridae) containing orthologs of the silkworm, Bombyx mori (Lepidoptera: Bombycidae).
Ohno M, Fujimoto T, Naito Y, Joraku A, Yasukochi Y, Sahara K
Applied Entomology and Zoology 55 ( 1 ) 159 - 174 2020年02月 [査読有り]
国際的学術誌 共著・分担
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Activity and inactivity of moth sex chromosomes in somatic and meiotic cells.
Traut W, Schubert V, Daliková M, Marec F, Sahara K
Chromosoma 128 ( 4 ) 533 - 545 2019年08月 [査読有り]
国際的学術誌 共著・分担
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Construction of a BAC library of Endoclita excrescens as a tool for comparative gene mapping in Lepidoptera.
Okumura A, Kobayashi M, Kitajima H, Yasukochi Y, Suzuki G, Sahara K
Entomological Science 22 167 - 172 2019年06月 [査読有り]
学会誌 共著・分担
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Dimorphic sperm formation by Sex-lethal.
Hiroki Sakai, Hiroyuki Oshima, Kodai Yuri, Hiroki Gotoh, Takaaki Daimon, Toshinobu Yaginuma, Ken Sahara, and Teruyuki Niimi
Proceedings National Academy of Sciences USA 116 ( 21 ) 10412 - 10417 2019年05月 [査読有り]
学術誌 共著・分担
Sex is determined by diverse mechanisms and master sexdetermination
genes are highly divergent, even among closely
related species. Therefore, it is possible that homologs of master
sex-determination genes might have alternative functions in
different species. Herein, we focused on Sex-lethal (Sxl), which is
the master sex-determination gene in Drosophila melanogaster
and is necessary for female germline development. It has been
widely shown that the sex-determination function of Sxl in Drosophilidae
species is not conserved in other insects of different
orders. We investigated the function of Sxl in the lepidopteran
insect Bombyx mori. In lepidopteran insects (moths and butterflies), spermatogenesis results in two different types of sperm: nucleated fertile eupyrene sperm and anucleate nonfertile parasperm, also known as apyrene sperm. Genetic analyses using Sxl mutants revealed that the gene is indispensable for proper morphogenesis of apyrene sperm. Similarly, our analyses using Sxl -
New Stigmaeopsis species on Miscanthus grasses in Taiwan and Thailand (Acari, Tetranychidae).
Saito Y, Sato Y, Kongchuensin M, Chao JT, Sahara K
Systematic and Applied Acarology 24 ( 4 ) 675 - 682 2019年04月 [査読有り]
国際的学術誌 共著・分担
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Unique sex determination system in the silkworm, Bombyx mori: current status and beyond.
Katsuma S, Kiuchi T, Kawamoto M, Fujimoto T, Sahara K
The Proceedings of the Japan Academy, Series B 94 ( 5 ) 205 - 216 2018年05月 [査読有り]
国際的学術誌 共著・分担
(2018) The Proceedings of the Japan Academy, Series B 94 (5): 205-216.
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Fujimoto T, Okumura A, Yoshido A, Yasukochi Y, Suzuki G, Sahara K
. Journal of Insect Biotechnology and Sericology 87 ( 2 ) 61 - 69 2018年05月 [査読有り]
学会誌 共著・分担
We constructed the first trichopteran bacterial artificial chromosome (BAC) library in Stenopsyche marmorata. The BAC library consists of 32,256 clones with an average insert size of 65.38 kb. We also made a cDNA library from an S. marmorata adult male and determined 598 unique sequences. Using the obtained sequences we designed putative single tagged site (STS) primers and selected 81 BACs containing orthologs of Bombyx mori genes by three-step PCR selection method. These BACs will be used for gene-based comparative mapping by fluorescence in situ hybridization (FISH) to identify regions of conserved synteny between the trichopteran and lepidopteran genomes. -
Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of distantly related ditrysian Lepidoptera.
Yasukochi Y, Yang B, Fujimoto T, SaharaK, Matsuo T, Ishikawa Y
PLoS ONE 13 ( 2 ) e0192762 2018年02月 [査読有り]
国際的学術誌 共著・分担
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Inheritance of cocoon color mutation and heterosis in Antheraea yamamai.
Yamada Y, Asano S, Bando H, Sahara K
Journal of Wild Silkworm & Silk 20 57 - 61 2017年10月 [査読有り]
国際的学術誌 共著・分担
Antheraea yamamai is a wild silkmoth that spins yellow-green silk to produce cocoons. Cocoons that are less yellowish in color, referred to as emerald green (EG), have been found in A. yamamai mass-rearing for cocoon production. Here, we examined EG inheritance by crossing individuals that produce EG cocoons with those that produce normal-colored (normal green: NG) cocoons. F1 progenies from the reciprocal cross between EG and NG individuals mostly produced NG cocoons. In the backcross, color
segregation mostly indicated that the EG mutation is controlled by a single recessive gene locus. Single-pair selection of cocoon weight for 4 years revealed that there is a heterosis effect on cocoon weight in the hybrid
progenies. -
Kageyama D, Ohno M, Sasaki T, Yoshido A, Konagaya T, Jouraku A, Kuwazaki S, Kanamori H, Katayose Y, Narita S, Miyata M, Riegler M, Sahara K
Evolution Letters 1 ( 5 ) 232 - 244 2017年10月 [査読有り]
国際的学術誌 共著・分担
(2017): 1(5): 232-244.
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Relative humidity and maintenance of p50 silkworms reared on artificial diet.
Yuri K, Sahara K
Journal of Insect Biotechnology and Sericology 86 43 - 47 2017年04月 [査読有り]
学会誌 共著・分担
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A history of chromosome identification in Bombyx mori
Ken Sahara, Atsuo Yoshido, Yuji Yasukochi
Chromosome Science 19 3 - 9 2017年04月 [査読有り]
学会誌 共著・分担
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Variation in nesting behavior of eight species of spider mites, Stigmaeopsis having sociality
Yutaka Saito, Yan-Xuan Zhang, Kotaro Mori, Katsura Ito, Yukie Sato, Anthony R. Chittenden, Jian-Zhen Lin, Younghae Chae, Takane Sakagami, Ken Sahara
Science of Nature 103 87 2016年10月 [査読有り]
学術誌 共著・分担
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Fissions, fusions, and translocations shaped the karyotype and multiple sex chromosome constitution of the northeast-Asian wood white butterfly, Leptidea amurensis
JINDRA SICHOVA, MIZUKI OHNO, VLAD DINCA, MICHIHITO WATANABE, KEN SAHARA, FRANTISEK MAREC
Biological Journal of the Linnean Society 118 457 - 471 2016年05月 [査読有り]
学術誌 共著・分担
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The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction
A Yoshido, F Marec, K Sahara
Heredity 116 424 - 433 2016年04月 [査読有り]
学術誌 共著・分担
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XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima
Jack E. Green, Martina Dalíková, Ken Sahara, František Marec, Michael Akam
PLoSONE 11 ( 2 ) e0150292 2016年02月 [査読有り]
学術誌 共著・分担
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Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.
Ito K, Katsuma S, Kuwazaki S, Jouraku A, Fujimoto T, Sahara K, Yasukochi Y, Yamamoto K, Tabunoki H, Yokoyama T, Kadono-Okuda K, Shimada T
Heredity 116 52 - 59 2016年01月 [査読有り]
国際的学術誌 共著・分担
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A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm.
Yasukochi Y, Ohno M, Shibata F, Jouraku A, Nakano R, Ishikawa Y, Sahara K
Heredity 116 75 - 83 2016年01月 [査読有り]
国際的学術誌 共著・分担
(2016): Heredity 116, 75-83
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Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies.
Šíchová J, Voleníková A, Dincă V, Nguyen P, Vila R, Sahara K, Marec F
BMC Evolutionary Biology 15 ( 89 ) 2015年05月 [査読有り]
国際的学術誌 共著・分担
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A new molecular technique for determining the sex of Harmonia axyridis.
Gotoh H, Nishikawa H, Sahara K, Yaginuma T, Niimi T
Journal of Insect Biotechnology and Sericology 84 9 - 15 2015年02月 [査読有り]
学会誌 共著・分担
We have developed a fast and efficient PCR-based technique to sex all developmental stages of the ladybird beetle Harmonia axyridis. Previously, we established a male-specific fluorescent expressing strain of H. axyridis with the piggyBac vector. FISH analyses revealed that the transgene inserted into the H. axyridis genome in the male Y-chromosome. Because only males expressed the fluorescent signal, we were able to differentiate between the sexes of this transgenic strain even in newly hatched larvae. We took advantage of this insertion and designed an inverse PCR reaction and genome walking surrounding the inserted region to identify 3,781 bp of the male Y-chromosome genomic sequence. From this male specific region, we designed a PCR-based protocol to identify sexes from all H. axyridis developmental stages.
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FISH identification of Helicoverpa armigera and Mamestra brassicae chromosomes by BAC and fosmid probes.
Sahara K , Yoshido A, Shibata F, Fujikawa N, Okabe T, Tanaka-Okuyama M, Yasukochi Y
Insect Biochemistry and Molecular Biology in press 2013年04月 [査読有り]
国際的学術誌 共著・分担
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Neo-sex chromosomes and adaptive potential in tortricid pests.
Nguyen P, Sýkorová M, Šíchová J, Kůta V, Dalíková M, Čapková Frydrychová R, Neven LG, Sahara K, Marec F
The Proceedings National Academy of Sciences USA 110 6931 - 6936 2013年04月 [査読有り]
国際的学術誌 共著・分担
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Rapid turnover of the W chromosome in geographical populations of wild silkmoths, Samia cynthia ssp.
Yoshido A, Šíchová J, Kubíčková S, Marec F, Sahara K
Chromosome Research 21 149 - 164 2013年04月 [査読有り]
国際的学術誌 共著・分担
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EST sequencing and fosmid library construction in a non-model moth, Mamestra brassicae, for comparative mapping （訳）比較マッピングのための非モデルガ類のヨトウガにおけるEST配列決定とフォスミドライブラリー作製
Kamimura M, Taetishi K, Tanaka-Okuyama M, Okabe T, Shibata F, Sahara K, Yasukochi Y
Genome in press 2012年10月 [査読有り]
国際的学術誌 共著・分担
ガとチョウ類のFISHを通じた比較ゲノム解析を進めるため、重要な農業害虫であるヨトウガのフォスミドライブラリーを作製し、EST配列の決定からカイコ単一遺伝子のオルソログを含むヨトウガフォスミドを120個特定した。この研究ではcDNAの行列プールを用いることでEST配列決定の効率化を実現した。一部のフォスミドを用いたFISH解析よりカイコとのシンテニーが示された。
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Phenotypic grouping of 141 BmNPVs lacking viral gene sequences （訳）ウイルス遺伝子欠損による141BmNPV遺伝子の形質グルーピング
Ono C, Kamagata T, Taka H, Sahara K, Asano S, Bando H
Virus Research 165 ( 2 ) 197 - 206 2012年05月 [査読有り]
国際的学術誌 共著・分担
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Sex chromosome evolution in moths and butterflies （訳）ガとチョウ類における性染色体進化
Sahara K, Yoshido A, Traut W
Chromosome Research 20 ( 1 ) 83 - 94 2011年12月 [査読有り]
国際的学術誌 共著・分担
ガとチョウの仲間である鱗翅目昆虫の雌へテロ型(WZ/ZZ)性染色体進化についての総説。FISHによる性染色体同定、性染色体の由来、性染色体構成の多様性、性決定、性染色体上の遺伝子、遺伝子量補正、性染色体工学による応用ならびに展望に関して我々の研究データを中心に概説してある。
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Yasukochi Y, Miura N, Nakano R, Sahara K, Ishikawa Y
PLoS ONE 6 ( 4 ) e18843 2011年04月 [査読有り]
国際的学術誌 共著・分担
This is the first report of tandemly arrayed sex pheromone receptor genes in Lepidoptera. The localization of an OR gene cluster on the Z chromosome agrees with previous findings for a Z-linked locus responsible for O. nubilalis male behavioral response to sex pheromone. The 181-bp irect repeats might enhance gene duplications by unequal crossovers. An autosomal locus responsible for male esponse to sex pheromone in Heliothis virescens and H.subflexa was recently reported to contain at least four OR genes. Taken together, these findings support the hypothesis that eneration of additional copies of OR genes can increase the potential for male moths to acquire altered specificity for pheromone components, and accordingly, facilitate differentiation of sex pheromones.
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Samia cynthia versus Bombyx mori: Comparative gene mapping between a species with a low-number karyotype and the model species of Lepidoptera. （訳）シンジュサン対カイコ:鱗翅目昆虫における染色体数の少ない種とモデル種の比較遺伝子マッピング
Yoshido A, Yasukochi Y, Sahara K
Insect Biochemistry and Molecular Biology 41 370 - 377 2011年03月 [査読有り]
国際的学術誌 共著・分担
We performed gene-based comparative FISH mapping between a wild silkmoth, Samia cynthia ssp. with a low number of chromosomes (2n=25-28) and the model species, Bombyx mori (2n=56), in order to identify the genomic components that make up the chromosomes in a low-number karyotype. Mapping
of 64 fosmid probes containing orthologs of B. mori genes revealed that the homologues of either two or four B. mori chromosomes constitute the S. c. ricini (Vietnam opulation, 2n=27/28, Z0/ZZ) autosomes. Where tested, even the gene order was conserved between S. c. ricini and B. mori. This was also true for the originally autosomal parts of the neo-sex chromosomes in S. c. walkeri (Sapporo population, 2n=26/26, neo-Wneo-Z/neo-Zneo-Z) and S. cynthia subsp. indet. (Nagano population, 2n=25/26, neo-WZ1Z2/Z1Z1Z2Z2). The results are evidence for an internal stability of lepidopteran chromosomes even when all autosomes had undergone fusion processes to form a low-number karyotype. -
Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K
Journal of Biomedicine and Biotechnology ID 165894 2010年11月 [査読有り]
国際的学術誌 共著・分担
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran.
A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. -
Isolation of the Thosea asigna virus (TaV) from the epizootic Setothosea asigna larvae collected in South Sumatra and a study on its pathogenicity to Limacodidae larvae in Japan.（訳）南スマトラのSetothosea asigna集団感染個体からのThosea asigna virus (TaV) 分離と日本におけるイラガ科幼虫への感染性研究
Sugiharti M, Ono C, Ito T, Asano S, Sahara K, Yulia Pujiastuti Y, Bando H
Journal of Insect Biotechnology and Sericology 79 ( 3 ) 117 - 124 2010年11月 [査読有り]
学会誌 共著・分担
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Silk threads function as an “adhesive cleaner” for nest space in a social spider mite.（訳）社会性ハダニは絹糸を粘着クリーナーとして巣内を掃除する
Kanazawa M, Sahara K, Saito Y
Proceedings of the Royal Society B 278 ( 1712 ) 1653 - 1660 2010年11月 [査読有り]
国際的学術誌 共著・分担
Individuals of the social spider mite Stigmaeopsis longus live communally in narrow, humid nests made from silk threads and display nest sanitation behaviour through the coordinated deposition of faeces. We used artificial dust to experimentally determine that females of this species use silk threads to perform regular cleaning of the nest space and eggs. We first learned that silk-weaving behaviour is not a by-product of nest building (nest reinforcement); rather, it is actively performed as a function of cleaning the living space and eggs. Furthermore, we determined the effectiveness of the attending females by artificially manipulating their natural habitat, which is clearly connected to the cleaning behaviour by parental females. As such, we have uncovered an extraordinary new role of silk threads as devices
for cleaning the nest space and/or eggs. -
Step-by-step evolution of neo-sex chromosomes in geographical populations of wild silkmoths, Samia cynthia ssp.（シンジュサンの地域個体群に見られるネオ性染色体の順次進化）
Yoshido A, Sahara K, Marec F, Matsuda Y
Heredity 106 ( 4 ) 614 - 624 2010年07月 [査読有り]
国際的学術誌 共著・分担
Geographical subspecies of wild silkmoths, Samia cynthia
ssp. (Lepidoptera: Saturniidae), differ considerably in sex
chromosome constitution owing to sex chromosome fusions
with autosomes, which leads to variation in chromosome
numbers. We cloned S. cynthia orthologues of 16 Bombyx
mori genes and mapped them to chromosome spreads of
S. cynthia subspecies by fluorescence in situ hybridization
(FISH) to determine the origin of S. cynthia neo-sex
chromosomes. FISH mapping revealed that the Z chromosome
and chromosome 12 of B. mori correspond to the
Z chromosome and an autosome (A1) of S. c. ricini (Vietnam
population, 2n¼27, Z0 in female moths), respectively.
B. mori chromosome 11 corresponds partly to another
autosome (A2) and partly to a chromosome carrying
nucleolar organizer region (NOR) of this subspecies. The
NOR chromosome of S. c. ricini is also partly homologous to
B. mori chromosome 24. -
Intramolecular proteolytic nicking and binding of Bacillus thuringiensis Cry8Da toxin in BBMVs of Japanese beetle.（訳）マメコガネ中腸刷子縁膜小胞に対するBT Cry8Daトキシンのタンパク質分解性損傷と結合
Yamaguchi T, Sahara K, Bando H, Asano SI
Journal of Invertebrate Pathology 105 ( 3 ) 243 - 247 2010年07月 [査読有り]
国際的学術誌 共著・分担
Yamaguchi T, Sahara K, Bando H, Asano SI (2010): Intramolecular proteolytic nicking and binding of Bacillus thuringiensis Cry8Da toxin in BBMVs of Japanese beetle. J Invertebr Pathol. 105 (3), 243-247.
[Epub ahead of print] 2010 Jul 23 -
An HDAC inhibitor increases AcMNPV gene expression in mammalian cells. （訳）哺乳動物細胞ではＡｃＭＮＰＶ遺伝子発現がHDACを抑制する
Fujita R, Ohtsuka D, Sahara K, Asano S, Bando H
Archives of Virology 155 ( 4 ) 577 - 581 2010年02月 [査読有り]
国際的学術誌 共著・分担
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation for AcMNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, with implications for a mechanism of suppression of AcMNPV gene expression in BHK cells.
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Evolutionary dynamics of rDNA clusters on chromosomes of moths and butterflies (Lepidoptera).（訳）ガとチョウ類の染色体におけるrDNAクラスターのダイナミックな変化
Nguyen P, Sahara K, Yoshido A, Marec F
Genetica 138 ( 3 ) 343 - 345 2009年11月 [査読有り]
国際的学術誌 共著・分担
We examined chromosomal distribution of major ribosomal DNAs (rDNAs), clustered in the nucleolar organizer regions (NORs), in 18 species of moths and butterflies using fluorescence in situ hybridization with a codling moth (Cydia pomonella) 18S rDNA probe. Most species showed one or two rDNA clusters in their haploid karyotype but exceptions with 4–11 clusters also occurred. Our results
in a compilation with previous data revealed dynamic evolution of rDNA distribution in Lepidoptera except Noctuoidea, which showed a highly uniform rDNA pattern. In
karyotypes with one NOR, interstitial location of rDNA
prevailed, whereas two-NOR karyotypes showed mostly
terminally located rDNA clusters. A possible origin of the
single interstitial NOR by fusion between two NOR-hromosomes
with terminal rDNA clusters lacks support in available data. In some species, spreading of rDNA to new,
mostly terminal chromosome regions was found. -
Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping.（訳）遺伝子マッピングにより明らかになったカイコとタバコスズメガの広範な遺伝子シンテニー
Yasukochi Y, Tanaka-Okuyama M, Shibata F, Yoshido A, Marec F, Wu C, Zhang H-B, Goldsmith MR, Sahara K
PLoS ONE 4 ( 10 ) e7465 2009年10月 [査読有り]
国際的学術誌 共著・分担
(2009): Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping. PLoS ONE 4(10): e7465. doi:10.1371/journal.pone.0007465 +: the same contribution
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Takemura Y, Sahara K, Mochida Y, Ohnuma A
Applied Entomology and Zoology 44 ( 3 ) 413 - 417 2009年08月 [査読有り]
国際的学術誌 共著・分担
Feasible methods for sperm cryopreservation and subsequent artificial insemination are urgently needed to maintain
silkworm genetic resources with less laborious work and space cost. We have discovered that loss of the fertilizing
ability of cryopreserved diploid (p50) semen was restored by the addition of either fresh or frozen apyrene sperm
from triploid donors. To decrease usage of the amount of stored diploid semen, we monitored the effective ratio of
cryopreserved p50 semen and apyrene sperm from triploid males to restore fecundity. Restoration of the fertilizing
ability of cryopreserved p50 semen was kept by roughly one fourth decrease of the amount by increasing the apyrene
sperm ratio. Because semen collection from diploid males requires skillful and careful techniques, decreasing the
amount of cryopreserved semen may practically save laborious work. -
Molecular phylogeny of Stigmaeopsis, with special reference to speciation through host plant Shift.（訳）宿主植物のシフトを考慮したスゴモリはダニ属の分子系統
Sakagami T, Saito Y, Kongchuensin M, Sahara K
Annals of the Entomological Society of Ameria 102 ( 3 ) 360 - 366 2009年05月 [査読有り]
国際的学術誌 共著・分担
Similar nest-weaving habits and sociality characterize all the species belonging to the recently reinstated spider mite genus Stigmaeopsis. Molecular phylogenetic analysis using 28S rDNA shows that Stigmaeopsis forms a distinct clade and that there is another unrecognized clade involving
Schizotetranychus species with comparable nest-weaving habits distributing in tropical regions. Furthermore,
the phylogenic hypothesis suggests that Stigmaeopsis miscanthi (Saito) inhabiting Miscanthus sinensis Anderss. shared a common ancestor with Stigmaeopsis longus (Saito) inhabiting Sasa senanensis (Franch. et Sav.), both mite species having very similar social systems. We conÞrmed
reproductive isolation between them and tested the suitability of the two plant species as hosts for these two mite species. -
Shibata F, Sahara K, Naito Y, Yasukochi Y
Zoological Science 26 ( 3 ) 187 - 190 2009年03月 [査読有り]
国際的学術誌 共著・分担
Multicolor fluorescence in-situ hybridization (FISH) and subsequent reprobing of chromosome preparations increase the number of chromosomes and/or anchor loci on the chromosomes simultaneously identified. Reprobing techniques have been widely applied to chromosomes of vertebrates and plants. We have developed a novel reprobing protocol that utilizes multicolor FISH and bacterial artificial chromosome (BAC) probes to examine chromosome preparations in a model lepidopteran species, the silkworm, Bombyx mori. With standard two-color BAC-FISH, routinely used to map genes on B. mori chromosomes, we could localize only two probes on one preparation,
whereas our new protocol combining five-color BAC-FISH and preparation reprobing enabled us to simultaneously map 10 probes, as demonstrated with the Bombyx Z chromosome. The improved BAC-FISH technique will facilitate karyotyping and synteny analysis in Lepidoptera. -
Discovery of a novel Bacillus thuringiensis Cry8D protein and the unique toxicity of the Cry8D-class proteins against scarab beetles.（訳）新規Btタンパクの発見とCry8Dのコガネムシ類に対する特異な殺虫化性
Yamaguchi T, Sahara K, Bando H, Asano SI
Journal of Invetebrate Pathology 99 ( 3 ) 257 - 262 2008年06月 [査読有り]
国際的学術誌 共著・分担
A novel cry gene, cry8Db, highly toxic to scarab beetles such as the Japanese beetle, Popillia japonica Newman, was cloned from an isolate of Bacillus thuringiensis(Bt), BBT2-5. The cry8Db gene has 3525bp nucleotides and codes for a protein of 1174 amino acid residues. The protein, Cry8Db, has typical Bt characteristics such as the 8-block, conserved sequences and the three-domain 3D toxin structure as defined with Cry3Aa.The Cry8Db protein was assayed along with the other scarab active Cry8Da and Cry8Ca against the Japanese beetle. While Cry8Da and Cry8Db had toxicity against both adults and larvae of the Japanese beetle, Cry8Ca was toxic to only larvae. Cry8Ca showed no toxicity against the adult beetle up to 30 microg per 1 cm(2) of leaf discs on which the protein was applied.
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Sex chromosomes and sex determination in Lepidoptera （訳）鱗翅目昆虫における性染色体と性決定
Traut W, Sahara K, Marec F
Sexual Development 1 ( 6 ) 332 - 346 2008年01月 [査読有り]
国際的学術誌 共著・分担
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Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH.（訳）5色プローブBAC-FISHにより示されたカイコとタバコスズメガ第15染色体のシンテニー
Sahara K, Yoshido A, Marec F, Fuková I, Zhang H-B,Wu C, Goldsmith MR, Yasukochi Y
Genome 50 ( 11 ) 1061 - 1065 2007年11月 [査読有り]
国際的学術誌 共著・分担
The successful assignment of the existing genetic linkage groups (LGs) to individual chromosomes and the second-generation linkage map obtained by mapping a large number of bacterial artificial chromosome (BAC) contigs in the silkworm, Bombyx mori, together with public nucleotide sequence databases, offer a powerful tool for the study of synteny between karyotypes of B. mori and other Lepidopteran species. Conserved synteny of genes between particular chromosomes can be identified by comparatively mapping orthologous genes of the corresponding linkage groups with the help of BAC-FISH (fluorescent in situ hybridization). To achieve higher-throughput comparative mapping using BAC-FISH in Lepidoptera, we developed a protocol for five-color BAC-FISH, which allowed us to map simultaneously 6 different BAC probes to chromosome 15 in B. mori. All 6 M. sexta BAC clones hybridized to a single M. sexta bivalent in pachytene spermatocytes.
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Detection of gamma-tubulin in mitotic division of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).（訳）カイコとバッタ類の体細胞分裂記におけるγチューブリン検出
Matsuda Y, Sahara K, Yasukochi Y, Yamashiki N
Zoological Science 24 ( 8 ) 781 - 786 2007年08月 [査読有り]
国際的学術誌 共著・分担
We detected a putative gamma-tubulin gene in silico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. Gamma-tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper.
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Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter.（訳）核多核体病ウイルスのie1遺伝子に認められるエクダイソン応答配列
Kojima K, Oritani K, Nakatsukasa T, Asano S, Sahara K, Bando H.
Virus Research 130 ( 43832 ) 202 - 209 2007年07月 [査読有り]
国際的学術誌 共著・分担
A computer-assisted analysis identified tentative target sequences for regulatory proteins including ecdysone-inducible factors such as BmFTZ-F1 and Broad-Complex Z4 (BR-C Z4) in the ie1 promoter of BmNPV. A transient expression experiment using BmN cells and a series of truncated ie1 promoter constructs demonstrated that the activity of the ie1 promoter responded to alpha-ecdysone and 20-hydroxyecdysone, which required a tridecameric nucleotide stretch (ie1EcRE, 5'-GTGTTATCGACCT-3') homologous to the ecdysone response element reported for Drosophila (DmEcRE). RT-PCR demonstrated the expression of BmEcR and BmUSP, which are required as ecdysone-specific activators for EcRE-mediated activation, in BmN cells. Furthermore, the ie1 EcRE-mediated response was confirmed by using a recombinant BmNPV possessing a luciferase gene under the control of the ie1 promoter with or without ie1 EcRE. This is the first report of an ecdysone response element in a baculoviral gene promoter.
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Yoshido A, Yasukochi Y, Marec F, Abe H, Sahara K
Journal of Insect Biotechnology and Sericology 76 ( 1 ) 1 - 7 2007年02月 [査読有り]
学術誌 共著・分担
We newly isolated seven W chromosome derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by means of six different STS primer sets, which amplify each different female specific DNA fragment. Two color fluorescence in situ hybridization (FISH) with 19L6H W-BAC probe as a standard W-chromosome painter disclosed that six out of seven new W-BAC probes strongly highlighted the whole W chromosome in B. mori. Besides the W, hybridization signals occurred in telomeric and/or subtelomeric regions of the autosomes. The remaining W-BAC, 20H9D, provided discontinuous signals, which appeared in both terminal and central compartments of the W chromosome. A representative of W-chromosome painting BACs, 19L6H, and 20H9D W-BAC were hybridized to chromosome spreads of Bombyx mandarina females. The 19L6H W-BAC bound to the whole B. mandarina W chromosome as in B. mori.
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Yoshido A, Yamada Y, Sahara K
Journal of Insect Biotechnology and Sericology 75 ( 3 ) 141 - 151 2006年10月 [査読有り]
学術誌 共著・分担
We newly identified the W chromosome in eight lepidopteran species and confirmed the absence of W chromosome in Caligula japonica females with the Z0 sex chromosome constitution by means of genomic in situ hybridization (GISH). In pachytene oocytes of species with the WZ/ZZ sex chromosome system, female-derived genomic probes of the respective species highlighted the whole W chromosome thread in the WZ bivalent and also stained specific autosomal region(s) with telomeric and/or subtelomeric signals. On the basis of GISH results obtained in this study and earlier published, we classified karyotypes of lepidopteran species into three types.
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Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitecidal strain, Bacillus thuringiensis subsp. entomocidus INA288.（訳）強い蚊類殺虫活性をもつBT亜種entomocidus INA288由来の2つの結晶タンパク質遺伝子のクローニングと発現
Ito T, Ikeya T, Sahara K, Bando H, Asano S
Applied and Environmental Microbiology 72 ( 8 ) 5673 - 5676 2006年08月 [査読有り]
国際的学術誌 共著・分担
Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.
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Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori.（訳）無核精子は凍結保存したカイコ精液の受精能力を回復させる
Takemura Y, Sahara K, Mochida Y, Ohnuma A
Journal of Insect Physiology 52 ( 10 ) 1021 - 1026 2006年07月 [査読有り]
国際的学術誌 共著・分担
Female moths of Bombyx mori were artificially inseminated with cryopreserved semen. The fertility of inseminated females varied from 0% to 76.9% depending on the strain. Addition of fresh semen from triploid males, which are infertile but whose semen includes intact apyrene sperm, greatly improved fecundity of cryopreserved semen from normal males. Frozen apyrene sperm from the triploid donors also improved the fecundity of females, inseminated with cryopreserved normal semen, but less than fresh semen from triploid males. Fertilization success in B. mori requires the presence of both, intact eupyrene and apyrene sperm. Our results show that eupyrene sperm tolerate the cryopreservation process better than apyrene sperm. Hence, we recommend to add apyrene sperm from the triploid donors as helper sperm routinely to cryopreserved semen in artificial insemination. This may advance the application of cryopreservation as a routine technique to maintain silkworm resources.
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A second generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects.（訳）第二世代のカイコ分子連関地図は鱗翅目昆虫間の遺伝子配置の保存性とシンテニーを明らかとした
Yasukochi Y, Ashakumary LA, Baba K, Yoshido A, Sahara K
Genetics 173 ( 3 ) 1319 - 1328 2006年03月 [査読有り]
国際的学術誌 共著・分担
A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCR-based screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.
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Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.（訳）カイコSxl遺伝子のクローニングとマッピング
Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W
Genome 49 ( 3 ) 263 - 268 2006年03月 [査読有り]
国際的学術誌 共著・分担
We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.
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Phylogeny of the sex determining gene Sex-lethal in insects.（訳）昆虫性決定遺伝子Sxlの系統関係
Traut W, Niimi T, Ikeo K, Sahara K
Genome 49 ( 3 ) 254 - 262 2006年03月 [査読有り]
国際的学術誌 共著・分担
SXL gene has acquired a pivotal role in the sex-determining pathway of Drosophila, although it does not act as a sex determiner in non-drosophilids. The SXL protein has moderately conserved N- and C-terminal regions and a well-conserved central region including 2 RNA recognition motifs. Our phylogenetic analysis shows that a single orthologue of the Drosophila Sex-lethal (Sxl) gene is present in the genomes of the malaria mosquito Anopheles gambiae, the honeybee Apis mellifera, the silkworm Bombyx mori, and the red flour beetle Tribolium castaneum. The D. melanogaster, D. erecta, and D. pseudoobscura genomes, however, contain 2 paralogous genes, Sxl and CG3056, which are orthologous to the Anopheles, Apis, Bombyx, and Tribolium Sxl. Hence, a duplication in the fly clade generated Sxl and CG3056.
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Expression of Autographa californica multiple nucleopolyhedrovirus genes in mammalian cells and Upregulation of the host beta-actin gene.（訳）哺乳細胞における昆虫ウイルスAcMNPV遺伝子発現はβアクチン遺伝子発現を上昇させる
Fujita R, Matsuyama T, Yamagishi J, Sahara K, Asano S, Bando H
Journal of Virology 80 ( 5 ) 2390 - 2395 2006年03月 [査読有り]
国際的学術誌 共著・分担
The gene expression of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) was examined in two types of mammalian cells, human HeLa14 and hamster BHK cells. DNA microarray analysis followed by reverse transcription-PCR identified at least 12 viral genes transcribed in both HeLa14 cells and BHK cells inoculated with AcMNPV. 5' rapid amplification of cDNA ends was carried out to examine the transcriptional fidelity of these genes in HeLa14 cells. The transcription of ie-1, ie-0 and gp64 was initiated at a baculovirus early gene motif, CAGT, accompanied by a TATA motif. In addition, the same splicing observed for ie-0 mRNA in Sf9 cells occurred in HeLa14 cells. While the transcription initiation sites for pe38 and p6.9 were not located in the CAGT motif, most of them were in a typical eukaryotic RNA polymerase II promoter structure (a conventional TATA motif and/or an initiator).
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Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori.（訳）相互転座によるカイコW染色体の一部欠失
Abe H, Seki M, Ohbayashi F, Tanaka N, Yamashita J, Fujii T, Yokoyama T, Takahashi M, Banno Y, Sahara K, Yoshido A, Ihara J, Yasukochi Y, Mita K, Ajimura M, Suzuki MG, Oshiki T and Shimada T
Insect Molecular Biology 14 ( 4 ) 339 - 352 2005年08月 [査読有り]
国際的学術誌 共著・分担
In this study, we analysed the W chromosomal regions of the Zebra-W strain (T(W;3)Ze chromosome) and the Black-egg-W strain (T(W;10)+(w-2) chromosome) at the molecular level. Our results strongly indicate that the regions containing the W-Samurai and W-Mikan RAPD markers or the W-Mikan RAPD marker were deleted in the T(W;3)Ze and T(W;10)+(w-2) chromosomes, respectively, due to reciprocal translocation between the W chromosome and the autosome. This deletion apparently does not affect the expression of Fem; therefore, this deleted region of the W chromosome does not contain the putative Fem gene.
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Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG)n telomeric probe in some species of Lepidoptera.（訳）GISHとテロメアFISHを組み合わせた鱗翅目昆虫の性染色体構成判定
Yoshido A, Marec F and Sahara K
Chromosoma 114 ( 3 ) 193 - 202 2005年07月 [査読有り]
国際的学術誌 共著・分担
We have developed a simple method to resolve the sex chromosome constitution in females of Lepidoptera by using a combination of genomic in situ hybridization (GISH) and fluorescence in situ hybridization with (TTAGG)( n ) telomeric probe (telomere-FISH). In pachytene configurations of sex chromosomes, GISH differentiated W heterochromatin and telomere-FISH detected the chromosome ends. With this method we showed that Antheraea yamamai has a standard system with a fully differentiated W-Z sex chromosome pair. In Orgyia antiqua, we confirmed the presence of neo-W and neo-Z chromosomes, which most probably originated by fusion of the ancestral W and Z with an autosome pair. In contrast to earlier data, Orgyia thyellina females displayed a neo-ZW(1)W(2) sex chromosome constitution. The combination of GISH and telomere-FISH enabled us to acquire not only reliable information about sex chromosome constitution but also an insight into sex chromosome evolution in Lepidoptera.
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Inbreeding depression on female fecundity by genetic factors retained in small population of a male-haploid social mite.（訳）雄単数社会性ハダニの小さな個体群に認められた遺伝的要因による雌造卵数の近交弱性
Mori K, Saito Y, Sakagami T, Sahara K
Experimental and Applied Acarology 36 ( 43832 ) 15 - 23 2005年05月 [査読有り]
学会誌 共著・分担
We previously determined that certain recessive genes decrease female fecundity in a haplo-diploid spider mite, Stigmaeopsis miscanthi (Saito). However, whether the depression was caused by the breakdown of heterosis or the expression of deleterious genes retained in a population could not be determined, because we had started our inbreeding experiment from a mixture of two isolated populations. In order to answer this basic question, inbreeding effects on survival and fecundity were measured for eight small populations occurring far from the two initial populations. There was little depression of immature survival of inbred lineages in all populations. On the other hand, in two inbred lineages, both originating from the smallest populations, female oviposition decreased significantly with the increase of Wrights f-value, showing that mildly deleterious genes are actually retained even in natural populations of haplo-diploid organisms.
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Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus.（訳）核多核体病ウイルスに感染した昆虫細胞では植物ウイルスCMVの35Sプロモーター活性が上昇する
Abe T, Miyake N, Nishijima Y, Fujita R, Sahara K, Asano S, Bando H
Virus Research 112 ( 43832 ) 38 - 41 2005年04月 [査読有り]
国際的学術誌 共著・分担
We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.
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The Bombyx mori karyotype and the assignment of linkage groups.（訳）カイコにおけるカリオタイプと連関地図への統合
Yoshido A, Bando H, Yasukochi Y, Sahara K
Genetics 170 675 - 685 2005年03月 [査読有り]
国際的学術誌 共著・分担
Lepidopteran species have a relatively high number of small holocentric chromosomes (Bombyx mori, 2n = 56). Chromosome identification has long been hampered in this group by the high number and by the absence of suitable markers like centromere position and chromosome bands. In this study, we carried out fluorescence in situ hybridization (FISH) on meiotic chromosome complements using genetically mapped B. mori bacterial artificial chromosomes (BACs) as probes. The combination of two to four either green or red fluorescence-labeled probes per chromosome allowed us to recognize unequivocally each of the 28 bivalents of the B. mori karyotype by its labeling pattern. Each chromosome was assigned one of the already established genetic linkage groups and the correct orientation in the chromosome was defined. This facilitates physical mapping of any other sequence and bears relevance for the ongoing B. mori genome projects.
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Organization of the Hox gene cluster of the silkworm, Bombyx mori: a split of the Hox cluster in a non-Drosophila insect.（訳）カイコにおけるHOX遺伝子クラスター：非ショウジョウバエ昆虫におけるHOXクラスターの分断
Yasukochi Y, Ashakumary LA, Wu C, Yoshido A, Nohata J, Mita K, Sahara K
Development Genes and Evolution 214 ( 12 ) 606 - 614 2004年10月 [査読有り]
国際的学術誌 共著・分担
Yasukochi Y, Ashakumary LA, Wu C, Yoshido A, Nohata J, Mita K and Sahara K (2004): Organization of the Hox gene cluster of the silkworm, Bombyx mori: a split of the Hox cluster in a non-Drosophila insect. Dev. Genes Evol. 214 (12), 606-614.
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Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms.（訳）BmNPVへの耐性強化を目指したトランスジェニックカイコにおけるRNAi技術の応用
Isobe R, Kojima K, Matsuyama T, Quan G-X, Kanda T, Tamura T, Sahara K, Asano S-I, Bando H
Archives of Virology 149 ( 10 ) 1931 - 1940 2004年10月 [査読有り]
国際的学術誌 共著・分担
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Roles of actin networks in peristaltic squeezing of sperm bundles in Bombyx mori.（訳）カイコ精子束のスクイージングにおけるアクチンネットワークの役割
Sahara K and Kawamura N
Journal of Morphology 259 ( 1 ) 1 - 6 2004年01月 [査読有り]
国際的学術誌 共著・分担
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W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori.（訳）W由来のBACプローブはカイコにおけるW染色体とW染色体変異を特定する新たなツールである
Sahara K, Yoshido A, Kawamura N, Ohnuma A, Abe H, Mita K, Oshiki T, Shimada T, Asano S, Bando H, Yasukochi Y
Chromosoma 112 ( 1 ) 48 - 55 2003年06月 [査読有り]
国際的学術誌 共著・分担
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Application of artificial insemination technique to eupyrene and/or apyrene sperm in Bombyx mori.（訳）人工授精によるカイコの有核と無核精子の機能解析
Sahara K and Takemura Y
Journal of Experimental Zoology Part A-Comparative Experimental Biology 297A ( 2 ) 196 - 200 2003年06月 [査読有り]
国際的学術誌 共著・分担
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Moth sex chromatin probed by comparative genomic hybridization (CGH).（訳）CGHによるガ類Wクロマチンの比較
Sahara K, Marec F, Eickhoff U, Traut W
Genome 46 ( 2 ) 339 - 342 2003年04月 [査読有り]
国際的学術誌 共著・分担
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Function analysis of an immediate early gene, ie1, of Bombyx mori nucleopolyhedrovirus in mammalian cells.（訳）哺乳細胞におけるBmNPVのie1遺伝子機能解析
Matsuyama T, Asano S, Sahara K, Bando H
Journal of Insect Biotechnology and Sericology 72 ( 2 ) 87 - 94 2003年01月 [査読有り]
学会誌 共著・分担
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Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori.（訳）グルコースもしくはエクダイステロイド添加培養はカイコ無核精子の生産を増加させる
Kawamura N, Sahara K, Fugo H
Journal of Insect Physiology 49 ( 1 ) 25 - 30 2003年01月 [査読有り]
国際的学術誌 共著・分担
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Use of an N-terminal half truncated IE1 as an antagonist of IE1, an essential regulatory protein in baculovirus.（訳）バキュウロウイルスの必須調節因子であるIE1のアンタゴニストとしてのN末端部位の利用
Yamada Y, Matsuyama T, Quan GX, Kanda T, Tamura T, Sahara K, Asano S, Bando H
Virus Research 90 ( 43832 ) 253 - 261 2002年12月 [査読有り]
国際的学術誌 共著・分担
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Differences in diapause attributes between two forms distinguished by male-to-male aggression in a subsocial spider mite, Schizotetranychus miscanthi Saito.
Saito Y, Sakagami T, Sahara K
Ecological Research 17 ( 6 ) 645 - 653 2002年10月 [査読有り]
学会誌 共著・分担
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In vitro cultivation of spermatocysts to matured sperm in the silkworm, Bombyx mori.（訳）カイコ精母細胞から精子への体外培養
Kawamura N and Sahara K
Developmet Growth and Differentiation 44 ( 4 ) 273 - 280 2002年08月 [査読有り]
学会誌 共著・分担
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Cloning and expression of novel crystal protein genes cry39A and 39orf2 from Bacillus thuringiensis subsp. aizawai Bun1-14 encoding mosquitocidal proteins.（訳）Bacillus thuringiensis subsp. aizawai Bun1-14の持つ殺蚊性結晶タンパク質からの新規cry39Aと39orf2のクローニングと発現
Ito T, Sahara K, Bando H, Asano S
Journal of Insect Biotechnology and Sericology 71 ( 3 ) 123 - 128 2002年05月 [査読有り]
学会誌 共著・分担
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Double copulation of a female with sterile diploid and polyploid males recovers fertility in Bombyx mori.（訳）不妊2倍体と倍数体雄のに重複交尾によってカイコ雌の妊性が回復する
Sahara K and Kawamura N
Zygote 10 ( 1 ) 23 - 29 2002年02月 [査読有り]
国際的学術誌 共著・分担
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Antisense and double-strand RAN interferenece in a silkworm ovarian cell line.（訳）カイコ卵巣培養細胞におけるアンチセンスと二重鎖によるRNA阻害
Isobe R, Kojima K, Sahara K, Asano S, Bando H
Journal of Insect Biotechnology and Sericology 71 ( 1 ) 43 - 47 2001年12月 [査読有り]
学会誌 共著・分担
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Meiotic pairing of sex chromosome fragments and its relation to atypical transmission of a sex-linked marker in Ephestia kuehniella (Insecta, Lepidoptera).（訳）スジコナマダラメイガにおける性染色体断片の対合と伴性マーカーの非典型的な分離の関連性
Marec F, Tothov A, Sahara K, Traut W
Heredity 87 ( 12 ) 659 - 671 2001年12月 [査読有り]
国際的学術誌 共著・分担
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家蚕濃核病ウイルス (BmDNV-2)特異的DNAポリメラーゼ活性と末端配列認識タンパク質の解析.
小島 桂・平野 文・浅野真一郎・佐原 健・伴戸久徳
日本蚕糸学雑誌 70 ( 2 ) 103 - 108 2001年05月 [査読有り]
学会誌 共著・分担
小島 桂・平野 文・浅野真一郎・佐原 健・伴戸久徳(2001): 家蚕濃核病ウイルス (BmDNV-2)特異的DNAポリメラーゼ活性と末端配列認識タンパク質の解析. 日本蚕糸学雑誌, 70(2), 103-108.
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Significance of peristaltic squeezing of sperm bundles in the silkworm, Bombyx mori: Elimination of irregular eupyrene sperm nuclei of the triploid.（訳）カイコ精子束におけるスクイージングの意味：異常3倍体核の有核精子からの排除
Kawamura N, Yamashiki N, Saitoh H, Sahara K
Zygote 9 ( 2 ) 159 - 166 2001年05月 [査読有り]
国際的学術誌 共著・分担
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天蚕全齢人工飼料育に用いる飼料樹葉特性の検討.
佐原 健・斎藤 寛・菊池邦夫・山田恭裕・浅野眞一郎・伴戸久徳
日本応用動物昆虫学会誌 45 ( 2 ) 94 - 98 2001年03月 [査読有り]
学会誌 共著・分担
(2001): 天蚕全齢人工飼料育に用いる飼料樹葉特性の検討. 応用動物昆虫学雑誌, 45 (2), 94-98.
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The peristaltic squeezing of sperm bundles at the late stage of spermatogenesis in the silkworm, Bombyx mori.（訳）カイコ精子形成後期におけるスクイージング
Kawamura N, Yamashiki N, Saitoh H, Sahara K
Journal of Morphology 246 ( 2 ) 53 - 58 2000年10月 [査読有り]
国際的学術誌 共著・分担
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PfDNV遺伝子産物によるウイルスRNAスプライシングの抑制
山岸潤也・浅野真一郎・佐原 健・飯塚敏彦・伴戸久徳
日本蚕糸学雑誌 69 ( 4 ) 271 - 276 2000年04月 [査読有り]
学会誌 共著・分担
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Inbreeding depression by resessive deleterious genes affecting female fecandity of a haplo-diploid mite.（訳）劣性有害遺伝子による近交弱性は単数-2倍性のハダニ雌の生殖能力に影響を与える
Saito Y, Sahara K, Mori K
Journal of Evolution Biology 13 ( 4 ) 668 - 678 2000年03月 [査読有り]
国際的学術誌 共著・分担
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Analysis of proteins encoded in the bipartite genome of a new type of parvo-like virus isolated from silkworm. -Structual protein with DNA polymerase motifs-（訳）カイコから分離されたパルボウイルス様新規2断片ゲノムがコードするタンパク質の解析-DNAポリメラーゼモチーフを持つ構造タンパク質-
Hayakawa T, Kojima K, Nakagaki M, Sahara K, Asano S, Iizuka T, Bando H
Virus Research 66 ( 1 ) 101 - 108 1999年10月 [査読有り]
国際的学術誌 共著・分担
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TTAGG telomeric repeats in chromosomes of some insects and other arthropods.（訳）昆虫とその他節足動物における染色体のTTAGGテロメアリピート
Sahara K, Marec F, Traut W
Chromosome Research 7 ( 6 ) 449 - 460 1999年06月 [査読有り]
国際的学術誌 共著・分担
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Toxicity of Bacillus thuringiensis subsp. wuhanensis crystal protein to Bombyx mori and Spodoptera litura.（訳）カイコとハスモンヨトウ に対するBacillus thuringiensis subsp. wuhanensisの結晶タンパク質活性
Pujiastuti Y, Asano S, Sahara K, Bando H, Iizuka T
Journal of Sericultural Science Japan 68 ( 3 ) 195 - 199 1999年04月 [査読有り]
学会誌 共著・分担
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Molecular differentiation of sex chromosomes probed by comparative genomic hybridization.（訳）CGHにより検出した性染色体の分子的な分化
Traut W, Sahara K, Otto TD, Marec F
Chromosoma 108 ( 3 ) 173 - 180 1999年02月 [査読有り]
国際的学術誌 共著・分担
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Two clinal trends in male-male aggressiveness in a subsocial spider mite.（訳）亜社会性ハダニにおける雄間の攻撃性の２つの勾配傾向
Saito Y and Sahara K
Behavioral Ecology and Sociobiology 46 25 - 29 1998年12月 [査読有り]
国際的学術誌 共著・分担
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Spodoptera litura（ハスモンヨトウ）に殺虫活性を有するBacillus thuringiensis菌株におけるcry1遺伝子の同定
浅野真一郎・プジャスツティ ユリア・佐原 健・伴戸久徳・飯塚敏彦
日本蚕糸学雑誌 67 ( 3 ) 237 - 242 1998年03月 [査読有り]
学会誌 共著・分担
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Novel Bacillus thuringiensis serovar aizawai strains isolated from mulberry leaves in Indonesia.（訳）インドネシアの桑葉から分離された新規 Bacillus thuringiensis serovar aizawai株
Rizali A, Asano S, Sahara K, Band H, Lay BW, Hastowo S, Iiazuka T
Applied Entomology and Zoology 33 ( 1 ) 111 - 114 1997年09月 [査読有り]
学会誌 共著・分担
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A mehod for chromosomal DNA preparation from a small number of mites.（訳）少数のハダニか染色体DNAを抽出する方法
Sakagami T, Sahara K, Saito Y
Applied Entomology and Zoology 32 ( 3 ) 523 - 526 1997年09月 [査読有り]
学会誌 共著・分担
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Analysis of genetic information of an insect picorna-like virus, infectious flacherie virus of silkworm: evidence for evolutionary relationships among insect, mammalian and plant picorna(-like) viruses.（訳）昆虫ピコルナ様ウイルスのカイコIFVの遺伝情報解析：昆虫、哺乳類ならびに植物ピコルナ（様）ウイルス間の進化的相互関係の証明
Isawa H, AsanoS, Sahara K, Iizuka T, Bando H
Archives of Virology 143 ( 1 ) 127 - 143 1997年08月 [査読有り]
国際的学術誌 共著・分担
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PCR法を用いたカイコ病原ウイルス遺伝子の検出
景安聖士・早川 徹・伊澤晴彦・佐原 健・浅野真一郎・飯塚敏彦・伴戸久徳
日本蚕糸学雑誌 66 ( 6 ) 477 - 483 1997年06月 [査読有り]
学会誌 共著・分担
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こぶ(K)形質を持つ家蚕の卵サイズ
原 健・P. Rama Mohana Rao・山田恭裕・斉藤 寛・浅野真一郎・伴戸久徳・飯塚敏彦・中田 徹
日本蚕糸学雑誌 66 ( 5 ) 364 - 366 1997年05月 [査読有り]
学会誌 共著・分担
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家蚕4倍体雌雄相互交配によって得られたF14倍体の生存率ならびに卵の性状
佐原 健*・山田恭裕・斉藤 寛・中田 徹・浅野真一郎・伴戸久徳・川村直子・飯塚敏彦
日本蚕糸学雑誌 66 ( 5 ) 341 - 345 1997年05月 [査読有り]
学会誌 共著・分担
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Origin of the gene in the newly discovered large egg mutant of Bombyx mori.（訳）家蚕において新たに発見された大型卵突然変異
Sahara K, Kawamura N, Iizuka T, Doira H
Journal of Sericultural Science Japan 66 ( 4 ) 261 - 265 1997年04月 [査読有り]
学会誌 共著・分担