Affiliation |
IWATE University Faculty of Agriculture Department of Life Science Course of Molecular Biomedical Sciences |
Position |
Professor |
Tomokazu Fukuda
|
|
Research Interests 【 display / non-display 】
-
Livestock Science
-
Carcinogenesis
-
Radioactive Safety Evaluation
-
Critically Endangered Species
-
Immortalized Cell
Graduating School 【 display / non-display 】
-
-1999.03
Kobe University Faculty of Agriculture Graduated
Graduate School 【 display / non-display 】
-
-1999.03
Nara Medical University Graduate School, Division of Medicine Doctor's Course Completed
-
-1993.03
Kobe University Graduate School, Division of National Science and Technology Master's Course Completed
Degree 【 display / non-display 】
-
Nara Medical University - Ph.D. 1999.03.31
Campus Career 【 display / non-display 】
-
2017.05-Now
IWATE University Faculty of Agriculture Food Animal Medicine & Food Safety Research Center Professor [Concurrently]
-
2016.04-Now
IWATE University Faculty of Agriculture Veterinary Medicine Professor [Concurrently]
-
2016.04-Now
IWATE University Faculty of Science and Engineering Department of Chemistry and Biological Science Studies in Biological Sciences Professor [Duty]
External Career 【 display / non-display 】
-
2008.04-2016.03
Tohoku University Associate Professor
-
2005.05-2008.03
National Cancer Center Research Institute of Japan Technical Support Staff
-
2001.02-2005.04
National Institute of Environmental Health Sciences, NIEHS-NIH Visiting Fellow
-
1999.04-2001.01
Japanese Foundation of Cancer Research Technical Support Staff
-
1993.04-1995.03
生物環境科学研究所 Staff Scientist
Research Areas 【 display / non-display 】
-
Life Science / Animal production science
-
Life Science / Pathological biochemistry
-
Environmental Science/Agriculture Science / Conservation of biological resources
-
Environmental Science/Agriculture Science / Radiation influence
Recommended URL 【 display / non-display 】
-
Laboratory of Cell Engineering and Molecular Genetics
Course Subject 【 display / non-display 】
-
2020
Probability and Statistics
-
2020
Human Anatomy
-
2020
Biotechnology
-
2020
Biological Science and Engineering Laboratory 2
-
2020
Biological Science and Engineering Laboratory 1
Published Papers 【 display / non-display 】
-
Iwasaki A, Hatakeyama M, Liu Q, Orimoto A, Fukuda T, Kitaoka T
Carbohydr Polym ( Elsevier ) 359 2025.05 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
Human dental pulp stem cells (hDPSCs) are a promising cell source for tooth regeneration therapies. However, conventional culture scaffold materials are often animal-derived, leading to immunogenicity concerns and limited availability. In this study, we explored phosphorylated cellulose nanofibers (P-CNFs), which have a fine fiber morphology and phosphate groups, as a novel scaffold material for cell culture. Immortalized hDPSCs were cultured on P-CNF scaffolds with different phosphate contents (0-1.42 mmol g-1) prepared by varying the molar ratio of urea and diammonium hydrogen phosphate and the reaction time. Cells cultured on unmodified CNFs exhibited poor adhesion and formed spheroids, indicating low bioadaptability. In contrast, P-CNF scaffolds with moderate phosphate content (0.54-0.78 mmol g-1) significantly improved cell adhesion; further increases in phosphate content decreased cell adhesion, indicating a strong dependence on phosphate content. Intriguingly, even in the absence of differentiation inducers, hDPSCs on P-CNF scaffolds with an optimal phosphate content of 0.78 mmol g-1 showed equal or higher expression of hard tissue marker genes compared to collagen scaffolds with differentiation inducers, suggesting that P-CNFs can directly promote hard tissue differentiation. These findings highlight plant-derived, animal-free P-CNFs as a promising biomaterial for advanced dental tissue engineering.
-
Mitochondrial calpain-1 truncates ATP synthase beta subunit.
Chukai Y, Furukawa N, Kosegawa O, Bai L, Sugano E, Fukuda T, Tomita H, Ozaki T
Biochem Biophys Res Commun . ( Elsevier ) 765 151829 2025.04 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
-
Bai L, Wu T, Fukasawa M, Kashiwagi S, Tate H, Ozaki T, Sugano E, Tomita H, Ishii T, Akashi T, Fukuda T
Tissue Cell ( Elsevier ) 93 102631 2025.04 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
Testosterone signaling mediates diseases such as androgenetic alopecia and prostate cancer and is controlled by the activation of the androgen receptor (AR) and nuclear translocation of the ligand-receptor complex. This study established an immortalized dermal papilla cell line that stably expresses the AR labeled with a monomeric green fluorescence marker. The cells expressed the histone H2B protein as visualized using a red fluorescence marker, enabling the Detection of nuclear translocation under live cell conditions using image analysis. The AR was observed to be translocated from the cytoplasm to the nucleus of cells after stimulation with dihydrotestosterone (DHT). The signal intensity of the nuclear/cytoplasm ratio was analyzed using automatic image analysis and a newly developed algorithm. The quantitation method to detect nuclear translocation revealed that the AR nuclear signal plateaued approximately 20 min after DHT exposure. Our developed method has the potential to save human labor by the automatic process of the image.
-
DNA damage triggers the death of green sea turtle-derived cells at high temperature
Katayama M, Kondo S, Onuma M, Nakayama SMM, Fukuda T
Comp Biochem Physiol C Toxicol Pharmacol ( Elsevier ) 289 110127 2025.03 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
As temperatures rise due to increasingly severe global warming, the effect of high temperatures on wildlife, including green sea turtles, is one of the issues that must be addressed to ensure the conservation of biodiversity. In the current study, we found that green sea turtle cell death due to apoptosis occurred at 37 °C, which suppressed cell proliferation. We also found that high temperature-induced heat stress led to the accumulation of DNA damage in green sea turtle cells. DNA damage is a key factor for the induction of apoptosis. Therefore, heat stress-mediated DNA damage can trigger green sea turtle cell apoptosis. Based on these results, we predict that high temperatures, such as 37 °C, would adversely impact green sea turtles, resulting in death or health-related challenges. Importantly, this information can facilitate the conservation of green sea turtles because cellular and molecular studies regarding the effects of global warming effects remain limited.
-
Enhanced auditory responses in visual cortex of blind rats using intrinsic optical signal imaging
Maruoka S, Sugano E, Togawa R, Katayama N, Tabata K, Yoshizawa N, Morita R, Takita Y, Ozaki T, Fukuda T, Bai L, Tomita H
Scientific Reports ( Nature Springer ) 14 ( 1 ) 24740 2024.11 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
Functional maturation of the visual cortex is induced by visual experiences during critical periods. Blind animals and humans exhibit improved auditory abilities after losing their vision. Here we investigated the response of the visual cortex to white noise stimuli during the progression of photoreceptor degeneration in a rat model of blindness (Royal College of Surgeons [RCS] (rdy/rdy) rats). Optical coherence tomography of RCS (+/+) rats with normal visual function revealed normal photoreceptor cells, whereas 3-month-old RCS (rdy/rdy) rats demonstrated photoreceptor cell degeneration. Visual cortex responses (VCRs) to a single flash stimulus were negligible in 3-month-old photoreceptor-degenerated rats. However, VCRs with white noise stimuli were significantly increased in blind versus RCS rats (+/+). Slight changes in the intrinsic optical signals of the control rats were observed on the ventral side of the visual cortex. In contrast, responses were markedly increased throughout the visual cortex of RCS (rdy/rdy) rats. These results indicate that the visual cortex rapidly acquires auditory system function over the first 3 months of life and that the entire visual cortex, rather than just the portion close to the auditory cortex, responds to white noise.
Books 【 display / non-display 】
-
Masafumi Katayama, Tomokazu Fukuda ( Pages responsible : CHAPTER 8 Establishment of induced pluripotent stem cells from prairie vole-derived fibroblast )
Academic Press 2020.10 ISBN: I012822228X
Scholarly Book
-
Yasuyuki Abe, Hideaki Yamashiro, Yasushi Kino, Toshinori Oikawa, Masatoshi Suzuki, Yusuke Urushihara, Yoshikazu Kuwahara, Motoko Morimoto, Jin Kobayashi, Tsutomu Sekine, Tomokazu Fukuda, Emiko Isogai, and Manabu Fukumoto
Springer 2020.01
Scholarly Book
-
Banri Suzuki, Shigefumi Tanaka, Kohichi Nishikawa, Chikako Yoshida, Takahisa Yamada, Yasuyuki Abe, Tomokazu Fukuda, Jin Kobayashi, Gohei Hayashi, Masatoshi Suzuki, Yusuke Urushihara, Kazuma Koarai, Yasushi Kino, Tsutomu Sekine, Atsushi Takahasi, Toshihiro Shimizu, Hisashi Shinoda, Kazuki Saito, Emiko Isogai, Koh Kawasumi, Satoshi Sugimura, Hideaki Yamashiro, and Manabu Fukumoto
Springer 2020.01
Scholarly Book
-
Tomokazu Fukuda
Springer 2020.01
Scholarly Book
-
Motoko Morimoto, Ayaka Kato, Jin Kobayashi, Kei Okuda, Yoshikazu Kuwahara, Yasushi Kino, Yasuyuki Abe, Tsutomu Sekine, Tomokazu Fukuda, Emiko Isogai, and Manabu Fukumoto
Springer 2020.01
Scholarly Book
Review Papers 【 display / non-display 】
-
Japanese golden eagle conservation science: current status and future needs
Rob OGDEN, Tomokazu FUKUDA, Takayuki FUNO, Mamoru KOMATSU, Taku MAEDA, Anna MEREDITH, Masaya MIURA, Haruki NATSUKAWA, Manabu ONUMA, Yuki OSAFUNE, Keisuke SAITO, Yu SATO, Des THOMPSON, Miho INOUE-MURAYAMA
Japanese Journal of Zoo Wildlife Medicine ( Japanese Society of Zoo Wildlife Medicine ) 25 ( 1 ) 9 - 28 2020.03
Academic Journal
Presentations 【 display / non-display 】
-
Immortalization of primary cells derived from the endangered Ryukyu long-furred rat
Oral Presentation(General) Lanlan Bai, Noe Kikuchi, Takahiro Eitsuka, Himari Matsusaka, Kiyotaka Nakagawa,Masafumi Katayama, Keiko Ito, Miho Inoue-Murayama, Tohru Kiyono, Tomokazu Fukuda
JAACT 2024 Nagoya (Nagoya)
2023.11-2023.12Japanese Association for Animal Cell Technology
-
Application for iPS cell technology to avian species
Oral Presentation(Guest/Special) Katayama M, Fukuda T
JAACT 2023 Nagoya (Nagoya)
2023.11-2023.12Japanese Association for Animal Cell Technology
-
Increased lentivirus titer using ultra expression vectors
Poster (General) So Fujibayashi, Tohru Kiyono, Yuka Endo, Tetsuya Tani, Haruka Tate, Lanlan Bai,Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda
JAACT 2023 Nagoya (Nagoya)
2023.11-2023.12Japanese Association for Animal Cell Technology
-
Poster (General) Himari Matsusaka, Tao Wu, Tomoe Yamada Kato, Lanlan Bai, Hiroshi Tomita,Eriko Sugano, Taku Ozaki, Tohru Kiyono, Isao Okunishi, Tomokazu Fukuda
JAACT 2023 Nagoya (Nagoya)
2023.11-2023.12Japanese Association for Animal Cell Technology
-
Comparison of BLV infectivity between bovine leukemia virus susceptible and resistant cattle
Oral Presentation(General) Lanlan Bai, Liushiqi Borjigin, Hirotaka Sato, ShinNosuke Takeshima, Tomokazu Fukuda, Sakurako, Asaji, Asako Ando, Hidetoshi Inoko, Yoko Aida
2022.09
Academic Awards Received 【 display / non-display 】
-
English Presentation Award
2019.09.20
All winners: Tomokazu Fukuda
-
2017.03.30