Tomokazu Fukuda

写真a

Affiliation

IWATE University  Faculty of Agriculture  Department of Life Science  Course of Molecular Biomedical Sciences 

Position

Professor
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Research Interests 【 display / non-display 】

  • Livestock Science

  • Carcinogenesis

  • Radioactive Safety Evaluation

  • Critically Endangered Species

  • Immortalized Cell

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Graduating School 【 display / non-display 】

  • 　
    -
    1999.03

    Kobe University   Faculty of Agriculture   Graduated

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Graduate School 【 display / non-display 】

  • 　
    -
    1999.03

    Nara Medical University  Graduate School, Division of Medicine  Doctor's Course  Completed

  • 　
    -
    1993.03

    Kobe University  Graduate School, Division of National Science and Technology  Master's Course  Completed

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Degree 【 display / non-display 】

  • Nara Medical University -  Ph.D.  1999.03.31

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Campus Career 【 display / non-display 】

  • 2017.05
    -
    Now

    IWATE University   Faculty of Agriculture   Food Animal Medicine & Food Safety Research Center   Professor   [Concurrently]

  • 2016.04
    -
    Now

    IWATE University   Faculty of Agriculture   Veterinary Medicine   Professor   [Concurrently]

  • 2016.04
    -
    Now

    IWATE University   Faculty of Science and Engineering   Department of Chemistry and Biological Science   Studies in Biological Sciences   Professor   [Duty]

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External Career 【 display / non-display 】

  • 2008.04
    -
    2016.03

    Tohoku University   Associate Professor  

  • 2005.05
    -
    2008.03

    National Cancer Center Research Institute of Japan   Technical Support Staff  

  • 2001.02
    -
    2005.04

    National Institute of Environmental Health Sciences, NIEHS-NIH   Visiting Fellow  

  • 1999.04
    -
    2001.01

    Japanese Foundation of Cancer Research   Technical Support Staff  

  • 1993.04
    -
    1995.03

    生物環境科学研究所   Staff Scientist  

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Research Areas 【 display / non-display 】

  • Life Science / Animal production science

  • Life Science / Pathological biochemistry

  • Environmental Science/Agriculture Science / Conservation of biological resources

  • Environmental Science/Agriculture Science / Radiation influence

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Recommended URL 【 display / non-display 】

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Course Subject 【 display / non-display 】

  • 2020

    Probability and Statistics

  • 2020

    Human Anatomy

  • 2020

    Biotechnology

  • 2020

    Biological Science and Engineering Laboratory 2

  • 2020

    Biological Science and Engineering Laboratory 1

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Published Papers 【 display / non-display 】

  • Proliferation and differentiation of human dental pulp stem cells on phosphorylated cellulose nanofiber scaffolds

    Iwasaki A, Hatakeyama M, Liu Q, Orimoto A, Fukuda T, Kitaoka T

    Carbohydr Polym ( Elsevier )  359   2025.05  [Refereed]

    Bulletin of University, Institute, etc.  Multiple authorship

    Human dental pulp stem cells (hDPSCs) are a promising cell source for tooth regeneration therapies. However, conventional culture scaffold materials are often animal-derived, leading to immunogenicity concerns and limited availability. In this study, we explored phosphorylated cellulose nanofibers (P-CNFs), which have a fine fiber morphology and phosphate groups, as a novel scaffold material for cell culture. Immortalized hDPSCs were cultured on P-CNF scaffolds with different phosphate contents (0-1.42 mmol g-1) prepared by varying the molar ratio of urea and diammonium hydrogen phosphate and the reaction time. Cells cultured on unmodified CNFs exhibited poor adhesion and formed spheroids, indicating low bioadaptability. In contrast, P-CNF scaffolds with moderate phosphate content (0.54-0.78 mmol g-1) significantly improved cell adhesion; further increases in phosphate content decreased cell adhesion, indicating a strong dependence on phosphate content. Intriguingly, even in the absence of differentiation inducers, hDPSCs on P-CNF scaffolds with an optimal phosphate content of 0.78 mmol g-1 showed equal or higher expression of hard tissue marker genes compared to collagen scaffolds with differentiation inducers, suggesting that P-CNFs can directly promote hard tissue differentiation. These findings highlight plant-derived, animal-free P-CNFs as a promising biomaterial for advanced dental tissue engineering.

    DOI

  • Mitochondrial calpain-1 truncates ATP synthase beta subunit.

    Chukai Y, Furukawa N, Kosegawa O, Bai L, Sugano E, Fukuda T, Tomita H, Ozaki T

    Biochem Biophys Res Commun . ( Elsevier )  765   151829   2025.04  [Refereed]

    Bulletin of University, Institute, etc.  Multiple authorship

    DOI

  • Detection of the nuclear translocation of androgen receptor using quantitative and automatic cell imaging analysis

    Bai L, Wu T, Fukasawa M, Kashiwagi S, Tate H, Ozaki T, Sugano E, Tomita H, Ishii T, Akashi T, Fukuda T

    Tissue Cell ( Elsevier )  93   102631   2025.04  [Refereed]

    Bulletin of University, Institute, etc.  Multiple authorship

    Testosterone signaling mediates diseases such as androgenetic alopecia and prostate cancer and is controlled by the activation of the androgen receptor (AR) and nuclear translocation of the ligand-receptor complex. This study established an immortalized dermal papilla cell line that stably expresses the AR labeled with a monomeric green fluorescence marker. The cells expressed the histone H2B protein as visualized using a red fluorescence marker, enabling the Detection of nuclear translocation under live cell conditions using image analysis. The AR was observed to be translocated from the cytoplasm to the nucleus of cells after stimulation with dihydrotestosterone (DHT). The signal intensity of the nuclear/cytoplasm ratio was analyzed using automatic image analysis and a newly developed algorithm. The quantitation method to detect nuclear translocation revealed that the AR nuclear signal plateaued approximately 20 min after DHT exposure. Our developed method has the potential to save human labor by the automatic process of the image.

    DOI

  • DNA damage triggers the death of green sea turtle-derived cells at high temperature

    Katayama M, Kondo S, Onuma M, Nakayama SMM, Fukuda T

    Comp Biochem Physiol C Toxicol Pharmacol ( Elsevier )  289   110127   2025.03  [Refereed]

    Bulletin of University, Institute, etc.  Multiple authorship

    As temperatures rise due to increasingly severe global warming, the effect of high temperatures on wildlife, including green sea turtles, is one of the issues that must be addressed to ensure the conservation of biodiversity. In the current study, we found that green sea turtle cell death due to apoptosis occurred at 37 °C, which suppressed cell proliferation. We also found that high temperature-induced heat stress led to the accumulation of DNA damage in green sea turtle cells. DNA damage is a key factor for the induction of apoptosis. Therefore, heat stress-mediated DNA damage can trigger green sea turtle cell apoptosis. Based on these results, we predict that high temperatures, such as 37 °C, would adversely impact green sea turtles, resulting in death or health-related challenges. Importantly, this information can facilitate the conservation of green sea turtles because cellular and molecular studies regarding the effects of global warming effects remain limited.

    DOI

  • Enhanced auditory responses in visual cortex of blind rats using intrinsic optical signal imaging

    Maruoka S, Sugano E, Togawa R, Katayama N, Tabata K, Yoshizawa N, Morita R, Takita Y, Ozaki T, Fukuda T, Bai L, Tomita H

    Scientific Reports ( Nature Springer )  14 ( 1 ) 24740   2024.11  [Refereed]

    Bulletin of University, Institute, etc.  Multiple authorship

    Functional maturation of the visual cortex is induced by visual experiences during critical periods. Blind animals and humans exhibit improved auditory abilities after losing their vision. Here we investigated the response of the visual cortex to white noise stimuli during the progression of photoreceptor degeneration in a rat model of blindness (Royal College of Surgeons [RCS] (rdy/rdy) rats). Optical coherence tomography of RCS (+/+) rats with normal visual function revealed normal photoreceptor cells, whereas 3-month-old RCS (rdy/rdy) rats demonstrated photoreceptor cell degeneration. Visual cortex responses (VCRs) to a single flash stimulus were negligible in 3-month-old photoreceptor-degenerated rats. However, VCRs with white noise stimuli were significantly increased in blind versus RCS rats (+/+). Slight changes in the intrinsic optical signals of the control rats were observed on the ventral side of the visual cortex. In contrast, responses were markedly increased throughout the visual cortex of RCS (rdy/rdy) rats. These results indicate that the visual cortex rapidly acquires auditory system function over the first 3 months of life and that the entire visual cortex, rather than just the portion close to the auditory cortex, responds to white noise.

    DOI

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Books 【 display / non-display 】

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Review Papers 【 display / non-display 】

  • Japanese golden eagle conservation science: current status and future needs

    Rob OGDEN, Tomokazu FUKUDA, Takayuki FUNO, Mamoru KOMATSU, Taku MAEDA, Anna MEREDITH, Masaya MIURA, Haruki NATSUKAWA, Manabu ONUMA, Yuki OSAFUNE, Keisuke SAITO, Yu SATO, Des THOMPSON, Miho INOUE-MURAYAMA

    Japanese Journal of Zoo Wildlife Medicine ( Japanese Society of Zoo Wildlife Medicine )  25 ( 1 ) 9 - 28   2020.03

    Academic Journal  

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Presentations 【 display / non-display 】

  • Immortalization of primary cells derived from the endangered Ryukyu long-furred rat

    Oral Presentation(General)  Lanlan Bai, Noe Kikuchi, Takahiro Eitsuka, Himari Matsusaka, Kiyotaka Nakagawa,Masafumi Katayama, Keiko Ito, Miho Inoue-Murayama, Tohru Kiyono, Tomokazu Fukuda

    JAACT 2024 Nagoya  (Nagoya) 

    2023.11
    -
    2023.12

    Japanese Association for Animal Cell Technology

  • Application for iPS cell technology to avian species

    Oral Presentation(Guest/Special)  Katayama M, Fukuda T

    JAACT 2023 Nagoya  (Nagoya) 

    2023.11
    -
    2023.12

    Japanese Association for Animal Cell Technology

  • Increased lentivirus titer using ultra expression vectors

    Poster (General)  So Fujibayashi, Tohru Kiyono, Yuka Endo, Tetsuya Tani, Haruka Tate, Lanlan Bai,Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda

    JAACT 2023 Nagoya  (Nagoya) 

    2023.11
    -
    2023.12

    Japanese Association for Animal Cell Technology

  • Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

    Poster (General)  Himari Matsusaka, Tao Wu, Tomoe Yamada Kato, Lanlan Bai, Hiroshi Tomita,Eriko Sugano, Taku Ozaki, Tohru Kiyono, Isao Okunishi, Tomokazu Fukuda

    JAACT 2023 Nagoya  (Nagoya) 

    2023.11
    -
    2023.12

    Japanese Association for Animal Cell Technology

  • Comparison of BLV infectivity between bovine leukemia virus susceptible and resistant cattle

    Oral Presentation(General)  Lanlan Bai, Liushiqi Borjigin, Hirotaka Sato, ShinNosuke Takeshima, Tomokazu Fukuda, Sakurako, Asaji, Asako Ando, Hidetoshi Inoko, Yoko Aida

    2022.09
    　
    　

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Academic Awards Received 【 display / non-display 】

  • English Presentation Award

    2019.09.20

    All winners： Tomokazu Fukuda

  • 2017.03.30

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