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Affiliation |
IWATE University Faculty of Agriculture Department of Life Science Course of Molecular Biology and Biochemistry |
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Position |
Professor |
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Laboratory Address |
〒0208550 3-18-8, Ueda, Morioka |
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Laboratory Phone number |
+81-19-621-6147 |
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Laboratory Fax number |
+81-19-621-6147 |
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Mail Address |
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Ken Sahara
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Campus Career 【 display / non-display 】
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2012.04-Now
IWATE University Faculty of Agriculture Professor [Duty]
External Career 【 display / non-display 】
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2011.08-2012.03
Hokkaido University Lecturer
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2007.04-2011.07
Hokkaido University Assistant Professor
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1992.07-2007.03
Hokkaido University Research Assistant
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Plant protection science
Course Subject 【 display / non-display 】
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2018
Undergraduate Research
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2018
Introduction to Bioregulation
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2018
Introduction to Biological Control
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2018
Applied Entomology II
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2018
Applied Entomology
Published Papers 【 display / non-display 】
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Non-directional pairing of the WZ chromosome bivalent in Bombyx mori
Toshiaki Fujimoto, Susumu Katsuma, Ken Sahara
Journal of Insect Biotechnology and Sericology 94 19 - 22 2025.06 [Refereed]
Academic Journal Single Work
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Comprehensive genome annotation of Bombyx mori p50ma strain, a newly developed standard strain
Lee J, Fujimoto T, Sahara K, Toyoda A, Shimada T
Scientific Data ( Springer Nature ) 12 359 2025.02 [Refereed]
Academic Journal Single Work
B. mori is a model species of lepidopteran insects. The genome assembly has been successively updated
since the whole genome sequences were first determined in 2004. In addition, chromosome-scale
genome assemblies of not only standard strains but also practical strains have been reported. We
successfully constructed a chromosome-scale female genome assembly of p50ma, a standard strain
developed by Kyushu University. This assembly is now certified as a ‘reference’ in the NCBI datasets.
To improve the usability of this strain, here we report the achievement of gene model construction
based on the transcriptome information, followed by functional annotation. The assembly harbours
16,295 protein-coding genes. In addition, to improve gene knockout efficiency, we performed ATAC-seq
in early embryos and comprehensively identified open chromatin regions. Finally, small RNA-seq
(sRNA-seq) targeting PIWI-interacting RNA (piRNA) was performed in testes, ovaries, and early
embryos to identify piRNA clusters comprehensively. These data will increase the usability of p50ma as
a standard strain and facilitate NBRP users to exploit this strain. -
Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects
Jung Lee, Toshiaki Fujimoto, Katsushi Yamaguchi, Shuji Shigenobu, Ken Sahara, Toru Shimada
Scientific Data ( Springer Nature ) 12 124 2025.01 [Refereed]
Academic Journal Single Work
Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter
generation period than the prevailing model species, Bombyx mori, this species would be a novel
model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotation
information on the chromosome-scale assembly of T. varians previously published by our group. 9
RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction.
As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6%
of BUSCO sequences were present in our gene models. ATA C-seq was also conducted to determine
chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed T. varians
genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential
users who plan to use this species. -
錦秋×鍾和において5齢幼虫脱皮個体を得るアラテクトミー方法
KIM Jung, 金児 雄, 佐原 健,
東北蚕糸・昆虫利用研究報告 49 13 - 17 2024.12 [Refereed]
Others Multiple authorship
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Diogo Cavalcanti Cabral-de-Mello1, Atsuo Yoshido, Diogo Milani, Jindra Šíchová, Ken Sahara, František Marec
DNA Research ( Oxford University Press ) 2024.10 [Refereed]
Academic Journal Single Work
Books 【 display / non-display 】
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節足動物ゲノムにおける細胞遺伝学的マッピングプロトコル：第6章カイコ（鱗翅目昆虫）
Yoshido A, Sahara K, Yasukochi Y
CRC press 2014.11 ISBN: 978-146659815
Scholarly Book
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Natural History of Silk (in Japanese)
Saito Y, Sahara K eds
Kaiyu-sha 2012.09 ISBN: 9784905930860
Academic Books
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Molecular Biology and Genetics of the Lepidoptera
Goldsmith M, Marec F eds ( Pages responsible : Marec F, Sahara K, Traut W: Chapter 3: Rise and fall of the W chromosome in Lepidoptera )
CRC press 2009.09 ISBN: 978-1138111721
Academic Books
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Molecular Entomology (in Japanese)
Kamimura M, Hinomoto N, Kasai S, Takauchi H, Hatakeyama M, Ishibashi J eds.
Kyoritsu Shuppan 2009.08 ISBN: 9784320056954
Academic Books
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Future Biological Sciences: Listen from Insect
Hayashi Y, Ohtsuki Y, Matsumoto T, Masui H, Fujii H, Sugimura N, Yajima M, Furusawa T eds.
Kinugasa-Kai 2009.06
Academic Books
Presentations 【 display / non-display 】
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Silkworm BAC screening for construction of artificial chromosomes
Oral Presentation(General)
2020.12 -
BAC library construction and BAC-FISH mapping in the eastern pale clouded yellow butterfly, Colias erate
Oral Presentation(General) Mizuki Ohno, Toshiaki Fujimoto, Go Suzuki, Yuji Yasukochi, Ken Sahara
2020.01 -
A fate of sex chromosomes and its partners in sex-limited yellow cocoon (Sy) strain of Bombyx mori
Oral Presentation(General) Ken Sahara, Yukina Matsumoto, Atsuo Yoshido, Munetaka Kawamoto, Yuji Yasukochi, Yu Kaneko
2018.07 -
Sex chromosome evolution in Lepidoptera
Oral Presentation(Guest/Special) Ken Sahara
2016.09 -
Sex chromosome turnover in a wild silkmoth
Symposium, Workshop, Panelist (Public Offering) Ken Sahara, Atsuo Yoshido, Yuji Yasukochi
2015.04-2015.05
Academic Awards Received 【 display / non-display 】
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2016.03.17
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The prize for the Society of Chromosome Research
2015.04.29
All winners： Ken Sahara
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2014.10.31
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Japan Prize of Agricultural Sciences, Achievement Award for Young Scientists
2006.11.27
All winners： Sahara K
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Achievement Award for Young Scientists in Japanese Society of Sericultural Science
1997.04.01
All winners： Sahara K
Association Memberships 【 display / non-display 】
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2000.04
The Japanese Society for Wild Silkmoths