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• Mycobacterium avium subsp. paratuberculosis excreted in cattle feces shows higher resistance to disinfectants than cultured cells

  Shigeru Matsuzawa, Mari Takechi, Masatoshi Fujihara

  Japanese Journal of Veterinary Research ( Hokkaido University )  73 ( 3-4 ) 83 - 87   2025年12月  [査読有り]

  学術誌  共著・分担

  As Johne's disease progresses, clumps of Mycobacterium avium subsp. paratuberculosis (MAP) are identified in the feces. MAP shows resistance to disinfectants, and effective disinfection methods for MAP cells excreted in feces have not been verified. Furthermore, given that biofilm-like vesicles and spore-like morphotypes were observed in feces from infected cattle, we aimed to compare reduction rates between cultured and naturally infected MAP cells after disinfectant treatment. Although the logarithmic reduction value for all cultured MAP cells was > 3.0, four of 10 infected MAP cells showed values < 2.0 after dichloroisocyanuric acid treatment. Our findings suggest that materials contaminated with infected cattle feces should be exposed to higher concentrations of disinfectants than those reported to be effective based on cultured cells.

  DOI

• Identification of Escherichia coli flagellar antigen by disc immuno-immobilization

  Shiho Oyama, Masatoshi Fujihara

  The Journal of General and Applied Microbiology ( Applied Microbiology, Molecular and Cellular Biosciences Research Foundation )  70 ( 5 ) 246 - 248   2025年07月  [査読有り]

  学術誌  共著・分担

  Disc immuno-immobilization is a simple method for typing flagellar antigens from Salmonella enterica. In this study, we successfully adapted this method for Escherichia coli. All eleven strains tested were determined their antigens within 14 h from inoculation. This method improves the efficiency and speed, highlighting its usefulness in clinical laboratories.

  DOI