|
Affiliation |
IWATE University School of Veterinary Medicine Cooperative Department of Veterinary Medicine |
FUJIHARA Masatoshi
|
|
|
Published Papers 【 display / non-display 】
-
Mycobacterium avium subsp. paratuberculosis excreted in cattle feces shows higher resistance to disinfectants than cultured cells
Shigeru Matsuzawa, Mari Takechi, Masatoshi Fujihara
Japanese Journal of Veterinary Research ( Hokkaido University ) 73 ( 3-4 ) 83 - 87 2025.12 [Refereed]
Academic Journal Multiple authorship
As Johne's disease progresses, clumps of Mycobacterium avium subsp. paratuberculosis (MAP) are identified in the feces. MAP shows resistance to disinfectants, and effective disinfection methods for MAP cells excreted in feces have not been verified. Furthermore, given that biofilm-like vesicles and spore-like morphotypes were observed in feces from infected cattle, we aimed to compare reduction rates between cultured and naturally infected MAP cells after disinfectant treatment. Although the logarithmic reduction value for all cultured MAP cells was > 3.0, four of 10 infected MAP cells showed values < 2.0 after dichloroisocyanuric acid treatment. Our findings suggest that materials contaminated with infected cattle feces should be exposed to higher concentrations of disinfectants than those reported to be effective based on cultured cells.
-
Identification of Escherichia coli flagellar antigen by disc immuno-immobilization
Shiho Oyama, Masatoshi Fujihara
The Journal of General and Applied Microbiology ( Applied Microbiology, Molecular and Cellular Biosciences Research Foundation ) 70 ( 5 ) 246 - 248 2025.07 [Refereed]
Academic Journal Multiple authorship
Disc immuno-immobilization is a simple method for typing flagellar antigens from Salmonella enterica. In this study, we successfully adapted this method for Escherichia coli. All eleven strains tested were determined their antigens within 14 h from inoculation. This method improves the efficiency and speed, highlighting its usefulness in clinical laboratories.