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Affiliation |
IWATE University Faculty of Science and Engineering Department of Science and Engineering Materials Science Course |
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Position |
Associate Professor |
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Laboratory Address |
〒0200066 3-18-33, Ueda, Morioka |
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Laboratory Phone number |
+81-19-621-6311 |
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Homepage URL |
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Mail Address |
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SHIBA Yoko
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Research Interests 【 display / non-display 】
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ArfGAPs
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CD63, exosome
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membrane traffic
Graduating School 【 display / non-display 】
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-1111.11
University of Tsukuba Second Cluster of College Graduated
Graduate School 【 display / non-display 】
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-1111.11
University of Tsukuba Doctor's Course Completed
Degree 【 display / non-display 】
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University of Tsukuba - Doctor of Philosophy in Science 2004.03.25
Campus Career 【 display / non-display 】
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2025.04-Now
IWATE University Faculty of Science and Engineering Department of Physical Science and Materials Engineering Studies in Materials Science and Engineering Associate Professor [Duty]
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2016.05-2025.03
IWATE University Faculty of Science and Engineering Department of Chemistry and Biological Science Studies in Biological Sciences Associate Professor [Duty]
Message 【 display / non-display 】
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We are studying intracellular trafficking using mammalian cells. During my research in Japan, France, and the United States, I found that ArfGAP proteins, which were originally thought to simply inhibit membrane trafficking, can actively regulate cargo sorting and promote intracellular transport. our current research focuses on understanding how diverse exosome populations are generated and whether their formation pathways can be classified based on the functions of ArfGAP proteins.
More recently, we have become interested in developing technologies to artificially control intracellular cargoes and organelles. By combining cell biology with materials science and physics, we aim to establish the emerging field of organelle engineering, where organelles can be manipulated using magnetic nanoparticles, external magnetic fields, and physical energies such as plasma.
Course Subject 【 display / non-display 】
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2020
Introduction to project-based learning
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2020
Advanced Cell Biology
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2020
Graduation Research
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2020
English for Science 1
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2020
Regional Studies F
Published Papers 【 display / non-display 】
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Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles
Yuta Yonekawa, Kazuki Oikawa, Boldbaatar Bayarkhuu, Kizuna Kobayashi, Nana Saito, Ibuki Oikawa, Ryohei Yamada, Yu-han Chen, Koichi Oyanagi, Yuji Shibasaki, Satoru Kobayashi, Yoko Shiba
Cell Structure and Function 50 ( 1 ) 25 - 39 2025.02 [Refereed]
Academic Journal Multiple authorship
磁性ナノ粒子を乳がんの細胞に取り込ませ、磁場を外部から印加して初期エンドソームが特異的に損傷するか調べた論文。リソソームには膜損傷が起きず、初期エンドソーム特異的に損傷できることが明らかにした。
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ArfGAP2 promotes STING proton channel activity, cytokine transit, and autoinflammation
Subhajit Poddar , Samuel D. Chauvin , Christopher H. Archer , Wei Qian , Jean A. Castillo-Badillo, Xin Yin , W. Miguel Disbennett , Cathrine A. Miner , Joe A. Holley , Teresa V. Naismith , W. Alexander Stinson , Xiaochao Wei , Yue Ning, Jiayuan Fu , Trini A. Ochoa , Nehalee Surve , Shivam A. Zaver , Kimberly A. Wodzanowski, Katherine R. Balka, Rajan Venkatraman, Canyu Liu, Kelly Rome, Will Bailis, Yoko Shiba, Sara Cherry , Sunny Shin, Clay F. Semenkovich, Dominic De Nardo, Sunnie Yoh , Elisha D.O. Roberson, Sumit K. Chanda, David J. Kast, Jonathan J. Miner
Cell S0092-8674 ( 25 ) 00096 2025.02 [Refereed]
Academic Journal Multiple authorship
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Arf GTPase-activating proteins ADAP1 and ARAP1 regulate incorporation of CD63 in multivesicular bodies
Kasumi Suzuki, Yoshitaka Okawa, Sharmin Akter, Haruki Ito and Yoko Shiba
Biology Open 2024.04 [Refereed]
Academic Journal Multiple authorship
4開膜貫通タンパク質CD63はクラスリンアダプターAP-3に結合するが、エクソソームの積荷でもある。エクソソームは多胞体(MVB)が細胞膜と融合し内腔小胞(ILV)が輸送されたものと考えられる。CD63のMVBへの輸送に関与するArfGAPをスクリーニングしたところ、ADAP1とARAP1が見つかった。ADAP１とARAP1の欠損細胞ではMVBへ輸送されるEGFRの輸送には影響がなかったため、EGFRとCD63は異なるMVBに輸送され、ADAP1とARAP1はCD63の輸送にのみ関与することが明らかとなった。
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Fabrication of hyperbranched-polyglycidol-Fe3O4 nanocomposite labeled with fluorescein isothiocyanate via rapid ligand exchange reaction
Ayane Kawamura, Mirai Saijyo, Boldbaatar Bayarkhuu, Naoki Nishidate, Ibuki Oikawa, Satoru Kobayashi, Koichi Oyanagi, Yoko Shiba, Tadashi Tsukamoto, Yoshiyuki Oishi , Yuji Shibasaki
Polymer 2024.01 [Refereed]
Academic Journal Multiple authorship
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Arf GTPase-Activating proteins SMAP1 and AGFG2 regulate the size of Weibel-Palade bodies and exocytosis of von Willebrand factor
Asano Watanabe, Hikari Hataida, Naoya Inoue, Kosuke Kamon, Keigo Baba, Kuniaki Sasaki, Rika Kimura, Honoka Sasaki, Yuka Eura, Wei-Fen Ni, Yuji Shibasaki, Satoshi Waguri, Koichi Kokame and Yoko Shiba
Biology Open ( The company of Biologists ) 2021.09 [Refereed]
Bulletin of University, Institute, etc. Multiple authorship
止血因子vWFはクラスリンアダプターAP-1の欠損によって分泌顆粒」WPBへの輸送が阻害されることが知られる。vWFのWPBへの輸送に関与するArfGAPをスクリーニングしたところ、SMAP1とAGFG2が見つかった。SMAP1欠損ではWPBが粒状になるが、AGFG２欠損では逆にWPBが長くなり、vWFの分泌が阻害されることがわかった。
Books 【 display / non-display 】
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ER stress, secretory granule biogenesis and insulin
Michiko Saito and Yoko Shiba
IntechOpen 2018.11 ISBN: 978-1-78984-922-6
Scholarly Book
Presentations 【 display / non-display 】
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Elucidation of the Mechanism of CD63-Positive Multivesicular Body Formation
Oral Presentation(General) kasumi suzuki, yoko shiba
The 21st International Symposium on Biocontrol and Biotechnology / International Conference on Agriculture, Biology and Medical Sciences (ISBB2026/ICABM)
2026.05-2026.06 -
Functional Roles of ADAP1 and ARAP1 in the Anterograde Transport Pathway to Secretory Multivesicular Bodies
Poster (General) Ryosuke shiraita,Kasumi Suzuki, Shramin akter, Yahiko ogawa, Yoko Shiba
The 21st International Symposium on Biocontrol and Biotechnology / International Conference on Agriculture, Biology and Medical Sciences, ISBB2026/ICABM (Iwate university)
2026.05-2026.06 -
Effect of Low-temperature Plasma Treatment on the Infectivity of Lentivirus
Poster (General) Nana Saito, Yumeka Hashimoto, Katsuyuki Takahashi, Koichi Takaki, Jun-Seok Oh , Yoko Shiba
The 21st International Symposium on Biocontrol and Biotechnology / International Conference on Agriculture, Biology and Medical Sciences, ISBB2026/ICABM
2026.05-2026.06 -
Magnetically Induced Endosomal Escape Quantified by HaloTag® Ligand Functionalized Fluorescent Superparamagnetic Silica Nanoparticles
Poster (General) Kazuki Oikawa, Yoko Shiba
The 21st International Symposium on Biocontrol and Biotechnology / International Conference on Agriculture, Biology and Medical Sciences, ISBB2026/ICABM (Iwate university)
2026.05-2026.06 -
Identification of secretory MVBs incorporating cytosolic Ferritin and Membrane-Bound CD63
Oral Presentation(General) Sharmin Akter
The 21st International Symposium on Biocontrol and Biotechnology / International Conference on Agriculture, Biology and Medical Sciences, ISBB2026/ICABM, (Iwate university)
2026.05-2026.06
Academic Awards Received 【 display / non-display 】
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Excellent Oral presentation award
2026.05.31
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2026.05.31
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2026.05.31
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Excellent oral presentation Award
2025.12.13
All winners： Nana Saito, Yumeka Hashimoto, Katsuyuki Takahashi, Koichi Takaki, Jun-Seok Oh , Yoko Shiba
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2025.11.08
Association Memberships 【 display / non-display 】
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2015.04
Japanese Society for Cell Biology
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2018.11
Japanese Society on Thrombosis and Hemostasis
Academic Activity 【 display / non-display 】
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2024.04-2027.03
Japanese Society for Cell Biology