論文 - RAHMAN Abidur
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Rahman T, Shao M, Pahari S, Venglat P, Soolanayakanahally R, Qiu X, Rahman A, Tanino K
Dissecting the Roles of Cuticular Wax in Plant Resistance to Shoot Dehydration and Low-Temperature Stress in Arabidopsis ( International Journal of Molecular Science ) 22 ( 4 ) 1554 2021年02月 [査読有り]
学術誌 共著・分担
Cuticular waxes are a mixture of hydrophobic very-long-chain fatty acids and their derivatives accumulated in the plant cuticle. Most studies define the role of cuticular wax largely based on reducing nonstomatal water loss. The present study investigated the role of cuticular wax in reducing both low-temperature and dehydration stress in plants using Arabidopsis thaliana mutants and transgenic genotypes altered in the formation of cuticular wax. cer3-6, a known Arabidopsis wax-deficient mutant (with distinct reduction in aldehydes, n-alkanes, secondary n-alcohols, and ketones compared to wild type (WT)), was most sensitive to water loss, while dewax, a known wax overproducer (greater alkanes and ketones compared to WT), was more resistant to dehydration compared to WT. Furthermore, cold-acclimated cer3-6 froze at warmer temperatures, while cold-acclimated dewax displayed freezing exotherms at colder temperatures compared to WT. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis identified a characteristic decrease in the accumulation of certain waxes (e.g., alkanes, alcohols) in Arabidopsis cuticles under cold acclimation, which was additionally reduced in cer3-6. Conversely, the dewax mutant showed a greater ability to accumulate waxes under cold acclimation. Fourier Transform Infrared Spectroscopy (FTIR) also supported observations in cuticular wax deposition under cold acclimation. Our data indicate cuticular alkane waxes along with alcohols and fatty acids can facilitate avoidance of both ice formation and leaf water loss under dehydration stress and are promising genetic targets of interest.
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Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots
Aslam M, Kenji S, Qin Y, Rahman A
International Journal of Molecular Sciences ( MDPI ) 21 ( 22 ) 8441 2020年11月 [査読有り]
学術誌 共著・分担
The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators of plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA (miR) in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of Arabidopsis thaliana with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4 °C revealed that the auxin signaling mutant, solitary root 1 (slr1; mutation in Aux/IAA14), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRs in the wild-type and slr1 mutant roots using next-generation sequencing revealed 180 known and 71 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26–31 nt small RNA population in slr1 compared with wild type. Comparative analysis of microRNA expression shows significant differential expression of 13 known and 7 novel miRs in slr1 at 4 °C compared with wild type. Target gene expression analysis of the members from one potential candidate miR, miR169, revealed the possible involvement of miR169/NF-YA module in the Aux/IAA14-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRs in auxin and cold responses.
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Rahman A, Kawamura Y, Maeshima M, Rahman A, Uemura M
Plant and Cell Physiology ( Oxford Academic ) 61 ( 4 ) 787 - 802 2020年01月 [査読有り]
学術誌 共著・分担
Aquaporins play a major role in plant water uptake at both optimal and environmentally stressed conditions. However, the functional specificity of aquaporins under cold remains obscure. To get a better insight to the role of aquaporins in cold acclimation and freezing tolerance, we took an integrated approach of physiology, transcript profiling and cell biology in Arabidopsis thaliana. Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression, and immunoblotting analysis confirmed the increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation, suggesting that PIP2;5 plays a major role in tackling the cold milieu. Although single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance, they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type. Consistently, a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels. Collectively, our results suggest that aquaporin members including PIP1;4 and PIP2;5 function in concert to regulate cold acclimation and freezing tolerance responses.
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Li P, Yu Q, Xu G, Xu C, Qi S, Wang H, Zhong F, Baskin TI, Rahman A, Wu S
Current Biology 28 2777 - 2786 2018年09月 [査読有り]
国際的学術誌 共著・分担
The Casparian strip in the root endodermis forms an apoplastic barrier between vascular tissues and outer ground tissues to enforce selective absorption of water and nutrients. Because of its cell-type specificity, the presence of a Casparian strip is used as a marker for a functional endodermis. Here, we examine the minimal regulators required for reprograming non-endodermal cells to build a functional Casparian strip. We demonstrate that the transcription factor SHORT-ROOT (SHR) serves as a master regulator and promotes Casparian strip formation through two independent activities: inducing the expression of essential Casparian strip enzymes via MYB36 and directing the subcellular localization of Casparian strip formation via SCARECROW (SCR). However, this hierarchical signaling cascade still needs SHR-independent small peptides, derived from the stele, to eventually build a functional Casparian strip in non-endodermal cells.
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Li P, Yang M, Chang J, Wu J, Zhong F, Rahman A, Qin H, Wu S
Frontiers in Plant Science 9 ( 832 ) 1 - 15 2018年06月 [査読有り]
国際的学術誌 共著・分担
Casparian strip (CS) is an impregnation of endodermal cell wall, forming an apoplastic diffusion barrier which forces the symplastic and selective transport of nutrients across endodermis. This extracellular structure can be found in the roots of all higher plants and is thought to provide the protection of vascular tissues. In Arabidopsis, a genetic toolbox regulating the formation of Casparian strips has emerged recently. However, Arabidopsis has the stereotypical root which is much simpler than most other plant species. To understand the Casparian strip formation in a more complex root system, we examined CS regulatory pathways in tomato. Our results reveal a spatiotemporally conserved expression pattern of most essential components of CS machinery in tomato. Further functional analyses verify the role of homologous CS genes in the Casparian strip formation in tomato, indicating the functional conservation of CS regulatory cascade in tomato.
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Nakasone, A, Fujiwara, M, Fukao, Y, Biswas KK, Rahman A, Kawai-Yamada, M, Narumi I, Uchimiya H, and Oono Y
Plant Physiology 160 ( 1 ) 93 - 105 2012年05月 [査読有り]
国際的学術誌 共著・分担
Previously, a dysfunction of the SMALL ACIDIC PROTEIN1 (SMAP1) gene was identified as the cause of the anti-auxin resistant1 (aar1) mutant of Arabidopsis (Arabidopsis thaliana). However, the exact mechanism by which SMAP1 functions in auxin signaling remains unknown. Here, we demonstrate that SMAP1 is required for normal plant growth and development and the root response to indole-3-acetic acid or methyl jasmonate in the auxin resistant1 (axr1) mutation background. Deletion analysis and green fluorescent protein/glutathione S-transferase pull-down assays showed that SMAP1 physically interacts with the CONSTITUTIVE PHOTOMORPHOGENIC9 SIGNALOSOME (CSN) via the SMAP1 F/D region. The extremely dwarf phenotype of the aar1-1 csn5a-1 double mutant confirms the functional role of SMAP1 in plant growth and development under limiting CSN functionality.
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Shootward and rootward: peak terminology for plant polarity.
Baskin TI, Peret B, Baluška F, Benfey PN, Bennett M, Forde BG, Gilroy S, Helariutta Y, Hepler PK, Leyser O, Masson PH, Muday GK, Murphy AS, Poethig S, Rahman A, Roberts K, Scheres B, Sharp RE, and Somerville C
Trends in Plant Science 15 ( 11 ) 593 - 594 2010年10月 [査読有り]
学術誌 共著・分担
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PINOID kinase regulates root gravitropism through modulation of PIN2-dependent basipetal transport in Arabidopsis thaliana.
Sukumar P. Edwards KS, Rahman A, DeLong A, Muday GK
Plant Physiol ( 150 ) 722 - 735 2009年04月
学術誌 共著・分担
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Xu S, Rahman A, Baskin TI, Kieber JJ
Plant Cell 20 ( 11 ) 3065 - 3079 2008年04月 [査読有り]
学術誌 共著・分担
The plant cell wall is a dynamic structure that changes in response to developmental and environmental cues through poorly understood signaling pathways. We identified two Leu-rich repeat receptor-like kinases in Arabidopsis thaliana that play a role in regulating cell wall function. Mutations in these FEI1 and FEI2 genes (named for the Chinese word for fat) disrupt anisotropic expansion and the synthesis of cell wall polymers and act additively with inhibitors or mutations disrupting cellulose biosynthesis. While FEI1 is an active protein kinase, a kinase-inactive version of FEI1 was able to fully complement the fei1 fei2 mutant. The expansion defect in fei1 fei2 roots was suppressed by inhibition of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, an enzyme that converts Ado-Met to ACC in ethylene biosynthesis, but not by disruption of the ethylene response pathway. Furthermore, the FEI proteins interact directly with ACC synthase.
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Okamoto T, Tsurumi S, Shibasaki K, Obana Y, Takaji H, Oono Y, Rahman A
Plant Physiol. 146 ( 4 ) 1651 - 1662 2008年04月
学術誌 共著・分担
We investigated the role of ethylene and auxin in regulating the growth and morphology of roots during mechanical impedance by developing a new growing system and using the model plant Arabidopsis (Arabidopsis thaliana). The Arabidopsis seedlings grown horizontally on a dialysis membrane-covered agar plate encountered adequate mechanical impedance as the roots showed characteristic ethylene phenotypes: 2-fold reduction in root growth, increase in root diameter, decrease in cell elongation, and ectopic root hair formation. The root phenotype characterization of various mutants having altered response to ethylene biosynthesis or signaling, the effect of ethylene inhibitors on mechanically impeded roots, and transcription profiling of the ethylene-responsive genes led us to conclude that enhanced ethylene response plays a primary role in changing root morphology and development during mechanical impedance.
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Genetic Characterization of Mutants Resistant to the Antiauxin p-chlorophenoxyisobutyric Acid (PCIB) Reveals that AAR3, a Gene Encoding DCN1-Like Protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis roots.
Biswas KK, Ooura C, Higuchi K, Miyazaki Y, Nguyen VV, Rahman A, Uchimiya H, Kiyosue T, Koshiba T, Tanaka A, Narumi I, Oono Y
Plant Physiology ( 145 ) 773 - 785 2007年11月 [査読有り]
学術誌 共著・分担
To isolate novel auxin-responsive mutants in Arabidopsis (Arabidopsis thaliana), we screened mutants for root growth resistance to a putative antiauxin, p-chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action by interfering the upstream auxin-signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least five independent loci, including two known auxin-related loci, TRANSPORT INHIBITOR RESPONSE1 and Arabidopsis CULLIN1. antiauxin-resistant mutants (aars) aar3-1, aar4, and aar5 were also resistant to 2,4-dichlorophenoxyacetic acid as shown by a root growth assay. Positional cloning of aar3-1 revealed that the AAR3 gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DEFECTIVE IN CULLIN NEDDYLATION-1 protein through the DUF298 domain.
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Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation
Oikawa A, Rahman A, Yamashita T, Taira H, Kidou S
Journal of Experimental Botany ( 58 ) 2617 - 2625 2007年07月 [査読有り]
学術誌 共著・分担
P23k is a monocot-unique protein that is highly expressed in the scutellum of germinating barley seed.The role of P23k in barley physiology remains unclear. Here, to elucidate its physiological function, BSMV-based virus-induced gene silencing (VIGS) of P23k in barley leaves was performed. Expression and localization analyses of P23k mRNA in barley leaves showed up-regulation of P23k transcript with increased photosynthetic activity and the localization of these transcripts to the vascular bundles and sclerenchyma, where secondary wall formation is most active. VIGS of the P23k gene led to abnormal leaf development, asymmetric orientation of main veins, and cracked leaf edges caused by mechanical weakness. In addition, histochemical analyses indicated that the distribution of P23k in leaves coincides with the distribution of cell wall polysaccharides. Considering these results together, it is proposed that P23k is involved in the synthesis of cell wall polysaccharides.
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Auxin, actin, and growth of the Arabidopsis thaliana primary root.
Rahman A, Bannigan A, Sulaiman W, Oono Y, Baskin TI
The Plant Journal 50 ( 3 ) 514 - 528 2007年05月 [査読有り]
学術誌 共著・分担
To understand how auxin regulates root growth, we quantified cell division and elemental elongation, and examined actin organization in the primary root of Arabidopsis thaliana. In treatments for 48 h that inhibited root elongation rate by 50%, we find that auxins and auxin-transport inhibitors can be divided into two classes based on their effects on cell division, elongation and actin organization. Indole acetic acid (IAA), 1-naphthalene acetic acid (NAA) and tri-iodobenzoic acid (TIBA) inhibit root growth primarily through reducing the length of the growth zone rather than the maximal rate of elemental elongation and they do not reduce cell production rate. These three compounds have little effect on the extent of filamentous actin, as imaged in living cells or by chemical fixation and immuno-cytochemistry, but tend to increase actin bundling. Our results show that IAA regulates the size of the root elongation zone whereas 2,4-D affects cell production and actin-dependent processes.
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Rahman A, Nakasone A, Chhun T, Ooura C, Biswas KK, Uchimiya H, Tsurumi S, Baskin TI, Tanaka A, Oono Y
Plant Journal 47 ( 5 ) 788 - 801 2006年05月 [査読有り]
学術誌 共著・分担
2,4-dichlorophenoxyacetic acid (2,4-D), a chemical analogue of indole-3-acetic acid (IAA) is believed to share a common response pathway. Here, we show that a mutant, antiauxin resistant1 (aar1), identified in a screen for resistance to the anti-auxin p-chlorophenoxy-isobutyric acid (PCIB), is resistant to 2,4-D, yet nevertheless responds like the wild-type to IAA and 1-napthaleneacetic acid in root elongation and lateral root induction assays. That the aar1 mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the DR5:GUS and HS:AXR3NT-GUS backgrounds, as well as by real-time PCR quantification of IAA 11 expression. The two characterized aar1 alleles both harbor multi-gene deletions; however, 2,4-D responsiveness was restored by transformation with one of the genes SMAP1, which encodes a novel protein with unknown function.
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Structure-Function Analysis of the presumptive Arabidopsis Auxin Permease AUX1.
Swarup R, Kargul J, Marchant A, Zadik D, Rahman A, Mills R, Yemm A, May S, Williams L, Millner P, Tsurumi S, Moore I, Napier R, Kerr ID, Bennett MJ
Plant Cell 16 ( 11 ) 3069 - 3083 2004年10月 [査読有り]
学術誌 共著・分担
We have investigated the subcellular localization, the domain topology, and the amino acid residues that are critical for the function of the presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments and confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed that AUX1 colocalized with plasma membrane (PM) markers. Because of its PM localization, we were able to take advantage of the steep pH gradient that exists across the plant cell PM to investigate AUX1 topology using YFP as a pH-sensitive probe. The YFP-coding sequence was inserted in selected AUX1 hydrophilic loops to orient surface domains on either apoplastic or cytoplasmic faces of the PM based on the absence or presence of YFP fluorescence, respectively. We were able to demonstrate in conjunction with helix prediction programs that AUX1 represents a polytopic membrane protein composed of 11 transmembrane spanning domains.