Published Papers - Ken Sahara

人工飼料育によるボルバキア感染ならびに非感染キタキチョウの生長比較

佐原 健, 菊池 将騎, 吉田 理央, 飯沼 椋詞, 村山 蒼悟, 陰山 大輔

東北蚕糸・昆虫利用研究報告   48   22 - 25   2023.12  [Refereed]

Others  Multiple authorship

Male-killing virus in a noctuid moth Spodoptera litura

Keisuke Nagamine, Yoshiaki Kanno, Ken Sahara, Toshiaki Fujimoto, Atsuo Yoshido, Yukio Ishikawae, Misato Teraoa, Daisuke Kageyamab, Yoshinori Shintania

PNAS ( National Academy of Sciences of the United States of America )  120 ( 46 ) e2312124120 - e2312124120   2023.11  [Refereed]

Academic Journal  Multiple authorship

DOI

Lepidopteran Synteny Units reveal deep chromosomal conservation in butterflies and moths

Walther Traut, Ken Sahara, Richard H. ffrench-Constant

G3 ( Oxford )  13 ( 8 ) jkad134 - jkad134   2023.06  [Refereed]

Academic Journal  Multiple authorship

DOI

Wolbachi infection in Morioka population of Eurema mandaria

Takeuchi Y, Funaki H, Sahara K

  47   1 - 6   2022.12  [Refereed]

Others  Multiple authorship

Application of a BAC carrying 18S rDNA sequences found in a Pieris brassicae libirary

Yoda N, Fujimoto A, Sahara K

  47   7 - 10   2022.12  [Refereed]

Others  Multiple authorship

Another BAC library construction in Endoclita excrescens

Yoda N, Kitajima H, Suzuki G,Fujimoto A, Yasukochi Y, Sahara K

  46   1 - 5   2021.12  [Refereed]

Others  Multiple authorship

Maintenance of the Sxl mutant in Bombyx mori

Obara S, Takemura Y, Sakai H,Sahara K

  46   12 - 16   2021.12  [Refereed]

Others  Multiple authorship

Simple method to discriminate different types of mitochondrial sequences in Eurema mandarina

Takeuchi Y, Sahara K

  46   6 - 11   2021.12  [Refereed]

Others  Multiple authorship

A genome assemble trial using a trichopteran BAC-FISH

Fujimoto T, Ohbo M, Sahara K

  45   10 - 13   2020.12  [Refereed]

Others  Multiple authorship

Double-copulated introduction of ejaculate with dominant larval phenotype to maintain Bombyx mori mutant with dysfunctional apyrene sperm.

Sakai H, Konagaya T, Takemura Y, Sahara K, Niimi T

Journal of Insect Biotechnology and Sericology   89 ( 1 ) 39 - 43   2020.04  [Refereed]

Academic Journal  Single Work

Development of an application, Bombyx mori tool for ortholog picking 1 (BmTOP).

Ohno M, Kawamoto M, Sahara K

Journal of Insect Biotechnology and Sericology   89 ( 1 ) 1 - 7   2020.02  [Refereed]

International Conference Proceedings  Multiple authorship

BAC selection of the large white butterfly, Pieris brassicae (Lepidoptera: Pieridae) containing orthologs of the silkworm, Bombyx mori (Lepidoptera: Bombycidae).

Ohno M, Fujimoto T, Naito Y, Joraku A, Yasukochi Y, Sahara K

Applied Entomology and Zoology   55 ( 1 ) 159 - 174   2020.02  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

DOI

Activity and inactivity of moth sex chromosomes in somatic and meiotic cells.

Traut W, Schubert V, Daliková M, Marec F, Sahara K

Chromosoma   128 ( 4 ) 533 - 545   2019.08  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

DOI

Construction of a BAC library of Endoclita excrescens as a tool for comparative gene mapping in Lepidoptera.

Okumura A, Kobayashi M, Kitajima H, Yasukochi Y, Suzuki G, Sahara K

Entomological Science   22   167 - 172   2019.06  [Refereed]

International Conference Proceedings  Multiple authorship

DOI

Dimorphic sperm formation by Sex-lethal.

Hiroki Sakai, Hiroyuki Oshima, Kodai Yuri, Hiroki Gotoh, Takaaki Daimon, Toshinobu Yaginuma, Ken Sahara, and Teruyuki Niimi

Proceedings National Academy of Sciences USA   116 ( 21 ) 10412 - 10417   2019.05  [Refereed]

Academic Journal  Multiple authorship

Sex is determined by diverse mechanisms and master sexdetermination
genes are highly divergent, even among closely
related species. Therefore, it is possible that homologs of master
sex-determination genes might have alternative functions in
different species. Herein, we focused on Sex-lethal (Sxl), which is
the master sex-determination gene in Drosophila melanogaster
and is necessary for female germline development. It has been
widely shown that the sex-determination function of Sxl in Drosophilidae
species is not conserved in other insects of different
orders. We investigated the function of Sxl in the lepidopteran
insect Bombyx mori. In lepidopteran insects (moths and butterflies), spermatogenesis results in two different types of sperm: nucleated fertile eupyrene sperm and anucleate nonfertile parasperm, also known as apyrene sperm. Genetic analyses using Sxl mutants revealed that the gene is indispensable for proper morphogenesis of apyrene sperm. Similarly, our analyses using Sxl

DOI

New Stigmaeopsis species on Miscanthus grasses in Taiwan and Thailand (Acari, Tetranychidae).

Saito Y, Sato Y, Kongchuensin M, Chao JT, Sahara K

Systematic and Applied Acarology   24 ( 4 ) 675 - 682   2019.04  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

DOI

Unique sex determination system in the silkworm, Bombyx mori: current status and beyond.

Katsuma S, Kiuchi T, Kawamoto M, Fujimoto T, Sahara K

The Proceedings of the Japan Academy, Series B　   94 ( 5 ) 205 - 216   2018.05  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

(2018) The Proceedings of the Japan Academy, Series B　94 (5): 205-216.

DOI

Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae).

Fujimoto T, Okumura A, Yoshido A, Yasukochi Y, Suzuki G, Sahara K

. Journal of Insect Biotechnology and Sericology   87 ( 2 ) 61 - 69   2018.05  [Refereed]

International Conference Proceedings  Multiple authorship


We constructed the first trichopteran bacterial artificial chromosome (BAC) library in Stenopsyche marmorata. The BAC library consists of 32,256 clones with an average insert size of 65.38 kb. We also made a cDNA library from an S. marmorata adult male and determined 598 unique sequences. Using the obtained sequences we designed putative single tagged site (STS) primers and selected 81 BACs containing orthologs of Bombyx mori genes by three-step PCR selection method. These BACs will be used for gene-based comparative mapping by fluorescence in situ hybridization (FISH) to identify regions of conserved synteny between the trichopteran and lepidopteran genomes.

Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of distantly related ditrysian Lepidoptera.

Yasukochi Y, Yang B, Fujimoto T, SaharaK, Matsuo T, Ishikawa Y

PLoS ONE   13 ( 2 ) e0192762   2018.02  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

DOI

Inheritance of cocoon color mutation and heterosis in Antheraea yamamai.

Yamada Y, Asano S, Bando H, Sahara K

Journal of Wild Silkworm & Silk   20   57 - 61   2017.10  [Refereed]

Bulletin of University, Institute, etc.  Multiple authorship

Antheraea yamamai is a wild silkmoth that spins yellow-green silk to produce cocoons. Cocoons that are less yellowish in color, referred to as emerald green (EG), have been found in A. yamamai mass-rearing for cocoon production. Here, we examined EG inheritance by crossing individuals that produce EG cocoons with those that produce normal-colored (normal green: NG) cocoons. F1 progenies from the reciprocal cross between EG and NG individuals mostly produced NG cocoons. In the backcross, color
segregation mostly indicated that the EG mutation is controlled by a single recessive gene locus. Single-pair selection of cocoon weight for 4 years revealed that there is a heterosis effect on cocoon weight in the hybrid
progenies.